Recombinant
RabMAb

Anti-CD3 epsilon antibody [E292] (ab32327)

Overview

  • Product name
    Anti-CD3 epsilon antibody [E292]
    See all CD3 epsilon primary antibodies
  • Description
    Rabbit monoclonal [E292] to CD3 epsilon
  • Host species
    Rabbit
  • Specificity
    ab32327 recognises CD3 espilon. The antibody does not cross-react with other subunits of CD3.
  • Tested applications
    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD3 epsilon. The exact sequence is proprietary.

  • Epitope
    ab32327 reacts with an epitope located in the cytoplasmic region of CD3 epsilon.
  • Positive control
    • Jurkat cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC 1/50.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC or IP.
  • Target

    • Function
      The CD3 complex mediates signal transduction.
    • Sequence similarities
      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Images

    • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD3 epsilon with unpurified ab32327 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Anti-CD3 epsilon antibody [E292] (ab32327) at 1/5000 dilution + Jurkat cell lysate

      Predicted band size: 23 kDa
      Observed band size: 23 kDa

    References

    ab32327 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Question
    Answer

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