Recombinant
RabMAb

Recombinant Anti-CD3 epsilon antibody [EP449E] (ab52959)

Rabbit recombinant monoclonal CD3 epsilon antibody [EP449E]. Validated in WB, IP, IHC and tested in Human. Cited in 10 publication(s). Independently reviewed in 1 review(s).

Overview

  • Product name

    Anti-CD3 epsilon antibody [EP449E]
    See all CD3 epsilon primary antibodies
  • Description

    Rabbit monoclonal [EP449E] to CD3 epsilon
  • Host species

    Rabbit
  • Specificity

    ab52959 reacts with CD3 epsilon. It does not cross-react with other subunits of CD3.
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CD3 epsilon aa 150-250. Derived from the cytoplasmic domain.

  • Positive control

    • Jurkat cell lysate and human spleen tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/250 - 1/500.
IP 1/50.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Images

  • All lanes : Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/1000 dilution (purified)

    Lane 1 : Jurkat( Human T cell leukemia T lymphocyte )membrane fraction cell lysate
    Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) without membrane fraction cell lysate
    Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 20 seconds


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST

  • ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.
  • ab52959 showing positive staining in Normal tonsil tissue.

  • ab52959 showing positive staining in Normal spleen tissue.

  • ab52959 showing negative staining in Normal brain tissue.

  • ab52959 showing negative staining in Normal kidney tissue.

  • ab52959 showing negative staining in Skeletal muscle tissue.

References

This product has been referenced in:

  • Schaadt NS  et al. Image analysis of immune cell patterns in the human mammary gland during the menstrual cycle refines lymphocytic lobulitis. Breast Cancer Res Treat N/A:N/A (2017). IHC-P ; Human . Read more (PubMed: 28444535) »
  • Grabner A  et al. Noninvasive Imaging of Acute Renal Allograft Rejection by Ultrasound Detection of Microbubbles Targeted to T-lymphocytes in Rats. Ultraschall Med 37:82-91 (2016). IHC-P . Read more (PubMed: 25919412) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (distal colon)
Specification
distal colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Isabel Romero Calvo

Verified customer

Submitted Jan 11 2013

Question
Answer

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