Recombinant
RabMAb

Anti-CD3 epsilon antibody [EP449E] (ab52959)

Reviews (1)Q&A (1)References (10)

Overview

  • Product name
    Anti-CD3 epsilon antibody [EP449E]
    See all CD3 epsilon primary antibodies
  • Description
    Rabbit monoclonal [EP449E] to CD3 epsilon
  • Host species
    Rabbit
  • Specificity
    ab52959 reacts with CD3 epsilon. It does not cross-react with other subunits of CD3.
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CD3 epsilon aa 150-250. Derived from the cytoplasmic domain.

  • Positive control
    • Jurkat cell lysate and human spleen tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/250 - 1/500.
IP 1/50.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    The CD3 complex mediates signal transduction.
  • Sequence similarities
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Images

    • Flow Cytometry - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Flow Cytometry - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      Human peripheral blood lymphocytes stained with ab52959 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab52959, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

    • Western blot - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Western blot - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/10000 dilution + Jurkat cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      ab52959 showing positive staining in Normal tonsil tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      ab52959 showing positive staining in Normal spleen tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      ab52959 showing negative staining in Normal brain tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      ab52959 showing negative staining in Normal kidney tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      ab52959 showing negative staining in Skeletal muscle tissue.

    References

    This product has been referenced in:
    • Schaadt NS  et al. Image analysis of immune cell patterns in the human mammary gland during the menstrual cycle refines lymphocytic lobulitis. Breast Cancer Res Treat N/A:N/A (2017). IHC-P ; Human . Read more (PubMed: 28444535) »
    • Grabner A  et al. Noninvasive Imaging of Acute Renal Allograft Rejection by Ultrasound Detection of Microbubbles Targeted to T-lymphocytes in Rats. Ultraschall Med 37:82-91 (2016). IHC-P . Read more (PubMed: 25919412) »
    See all 10 Publications for this product

    Publishing research using ab52959? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 epsilon antibody [EP449E]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (distal colon)
    Specification
    distal colon
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mrs. Isabel Romero Calvo

    Verified customer

    Submitted Jan 11 2013

    Question

    Antikörper, der humane T Zellen erkennt aber NICHT T Zellen aus der Ratte in IHC-P.

    Read More
    Answer

    Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblaetern mit mehr Informatinen zugelangen. ab32327 Vielen Dank für Ihren Anruf. Ich habe nun ein paar Antikörper für Sie herausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und für IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblättern mit mehr Informationen zugelangen. ab32327 Click here (or use the following: https://www.abcam.com/index.html?datasheet=32327). ab33524 Click here (or use the following: https://www.abcam.com/index.html?datasheet=33524). ab52959 Click here (or use the following: https://www.abcam.com/index.html?datasheet=52959). ab109531 Click here (or use the following: https://www.abcam.com/index.html?datasheet=109531). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder zu melden, falls Sie weitere Fragen haben. Click here (or use the following: https://www.abcam.com/index.html?datasheet=32327). ab33524 Click here (or use the following: https://www.abcam.com/index.html?datasheet=33524). ab52959 Click here (or use the following: https://www.abcam.com/index.html?datasheet=52959). ab109531 Click here (or use the following: https://www.abcam.com/index.html?datasheet=109531). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zoegern Sie nicht, sich wioeder zu melden, falls Sie weitere Fragen haben.

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