Recombinant
RabMAb

Recombinant Anti-CD3 epsilon antibody [EP449E] (ab52959)

Rabbit recombinant monoclonal CD3 epsilon antibody [EP449E]. Validated in WB, IP, IHC and tested in Human. Cited in 10 publication(s). Independently reviewed in 1 review(s).

Reviews (1)Q&A (1)References (10)

Overview

  • Product name

    Anti-CD3 epsilon antibody [EP449E]
    See all CD3 epsilon primary antibodies

  • Description

    Rabbit monoclonal [EP449E] to CD3 epsilon

  • Host species

    Rabbit

  • Specificity

    ab52959 reacts with CD3 epsilon. It does not cross-react with other subunits of CD3.

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human CD3 epsilon aa 150-250. Derived from the cytoplasmic domain.

  • Positive control

    • Jurkat cell lysate and human spleen tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/50.

Target

  • Function

    The CD3 complex mediates signal transduction.

  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Images

    • Western blot - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Western blot - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      All lanes : Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/1000 dilution (purified)

      Lane 1 : Jurkat( Human T cell leukemia T lymphocyte )membrane fraction cell lysate
      Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) without membrane fraction cell lysate
      Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 23 kDa
      Observed band size: 23 kDa


      Exposure time: 20 seconds


      Blocking/Dilution buffer and concentration: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 showing positive staining in Normal tonsil tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 showing positive staining in Normal spleen tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 showing negative staining in Normal kidney tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 showing negative staining in Skeletal muscle tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Schaadt NS  et al. Image analysis of immune cell patterns in the human mammary gland during the menstrual cycle refines lymphocytic lobulitis. Breast Cancer Res Treat N/A:N/A (2017). IHC-P ; Human . Read more (PubMed: 28444535) »
    • Grabner A  et al. Noninvasive Imaging of Acute Renal Allograft Rejection by Ultrasound Detection of Microbubbles Targeted to T-lymphocytes in Rats. Ultraschall Med 37:82-91 (2016). IHC-P . Read more (PubMed: 25919412) »
    See all 10 Publications for this product

    Publishing research using ab52959? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 epsilon antibody [EP449E]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (distal colon)
    Specification
    distal colon
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mrs. Isabel Romero Calvo

    Verified customer

    Submitted Jan 11 2013

    Question

    Antikörper, der humane T Zellen erkennt aber NICHT T Zellen aus der Ratte in IHC-P.

    Read More
    Answer

    Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblaetern mit mehr Informatinen zugelangen. ab32327 Vielen Dank für Ihren Anruf. Ich habe nun ein paar Antikörper für Sie herausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und für IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblättern mit mehr Informationen zugelangen. ab32327 Click here (or use the following: https://www.abcam.com/index.html?datasheet=32327). ab33524 Click here (or use the following: https://www.abcam.com/index.html?datasheet=33524). ab52959 Click here (or use the following: https://www.abcam.com/index.html?datasheet=52959). ab109531 Click here (or use the following: https://www.abcam.com/index.html?datasheet=109531). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder zu melden, falls Sie weitere Fragen haben. Click here (or use the following: https://www.abcam.com/index.html?datasheet=32327). ab33524 Click here (or use the following: https://www.abcam.com/index.html?datasheet=33524). ab52959 Click here (or use the following: https://www.abcam.com/index.html?datasheet=52959). ab109531 Click here (or use the following: https://www.abcam.com/index.html?datasheet=109531). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zoegern Sie nicht, sich wioeder zu melden, falls Sie weitere Fragen haben.

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