For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    cd3-epsilon-antibody-ep449e-ab52959.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Adaptive Immunity T Cells CD
Share by email
RecombinantRabMAb

Recombinant Anti-CD3 epsilon antibody [EP449E] (ab52959)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (13)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
  • Western blot - Anti-CD3 epsilon antibody [EP449E] (ab52959)
  • Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] (ab52959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
  • Anti-CD3 epsilon antibody [EP449E] (ab52959)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP449E] to CD3 epsilon
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-CD4 antibody [EPR6855] (ab133616)
Protein
Product image
Recombinant human CD3 epsilon protein (Active) (ab167755)
Primary
Product image
Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)

View more associated products

Overview

  • Product name

    Anti-CD3 epsilon antibody [EP449E]
    See all CD3 epsilon primary antibodies
  • Description

    Rabbit monoclonal [EP449E] to CD3 epsilon
  • Host species

    Rabbit
  • Specificity

    ab52959 reacts with CD3 epsilon. It does not cross-react with other subunits of CD3.
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CD3 epsilon aa 150-250. Derived from the cytoplasmic domain.
    Database link: P07766

  • Positive control

    • Jurkat cell lysate and human spleen tissue. IP: Jurkat whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP449E
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage

Associated products

  • Alternative Versions

    • Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
    • Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat membrane extract lysate (ab30130)
  • Recombinant Protein

    • Recombinant human CD3 epsilon protein (Active) (ab167755)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P (2)
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/20.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/20.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P07766 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 916 Human
    • Omim: 186830 Human
    • SwissProt: P07766 Human
    • Unigene: 3003 Human
    • Alternative names

      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Western blot - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      All lanes : Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/1000 dilution (purified)

      Lane 1 : Jurkat( Human T cell leukemia T lymphocyte )membrane fraction cell lysate
      Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) without membrane fraction cell lysate
      Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 23 kDa
      Observed band size: 23 kDa


      Exposure time: 20 seconds


      Blocking/Dilution buffer and concentration: 5% NFDM/TBST

    • Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      Purified ab52959 at 1/20 dilution (1µg) immunoprecipitating CD3 epsilon in Jurkat whole cell lysate.
      Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
      Lane 2 (+): ab52959 + Jurkat whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52929 in Jurkat whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 23 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 showing positive staining in Normal tonsil tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 showing positive staining in Normal spleen tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 showing negative staining in Normal kidney tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

      ab52959 showing negative staining in Skeletal muscle tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-CD3 epsilon antibody [EP449E] (ab52959)
      Anti-CD3 epsilon antibody [EP449E] (ab52959)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (13)

    Publishing research using ab52959? Please let us know so that we can cite the reference in this datasheet.

    ab52959 has been referenced in 13 publications.

    • Schaadt NS  et al. Graph-based description of tertiary lymphoid organs at single-cell level. PLoS Comput Biol 16:e1007385 (2020). PubMed: 32084130
    • Kim D  et al. Somatic mTOR mutation in clonally expanded T lymphocytes associated with chronic graft versus host disease. Nat Commun 11:2246 (2020). PubMed: 32382059
    • Yang X  et al. Misdiagnosis of primary pleural DLBCL as tuberculosis: A case report and literature review. Mol Clin Oncol 8:729-732 (2018). PubMed: 29732155
    • Schaadt NS  et al. Image analysis of immune cell patterns in the human mammary gland during the menstrual cycle refines lymphocytic lobulitis. Breast Cancer Res Treat N/A:N/A (2017). IHC-P ; Human . PubMed: 28444535
    • Grabner A  et al. Noninvasive Imaging of Acute Renal Allograft Rejection by Ultrasound Detection of Microbubbles Targeted to T-lymphocytes in Rats. Ultraschall Med 37:82-91 (2016). IHC-P . PubMed: 25919412
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 epsilon antibody [EP449E]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 17 2022

    Western blot abreview for Anti-CD3 epsilon antibody [EP449E]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (T-cells)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris HCl)
    Loading amount
    7.69 µg
    Specification
    T-cells
    Blocking step
    Licor intercept TBS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 13 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 epsilon antibody [EP449E]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (distal colon)
    Specification
    distal colon
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Mrs. Isabel Romero Calvo

    Verified customer

    Submitted Jan 11 2013

    Question

    Antikörper, der humane T Zellen erkennt aber NICHT T Zellen aus der Ratte in IHC-P.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 22 2011

    Answer

    Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblaetern mit mehr Informatinen zugelangen. ab32327 Vielen Dank für Ihren Anruf. Ich habe nun ein paar Antikörper für Sie herausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und für IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblättern mit mehr Informationen zugelangen. ab32327 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-E292-ab32327.html). ab33524 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-PS1-ab33524.html). ab52959 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-EP449E-ab52959.html). ab109531 Click here (or use the following: https://www.abcam.com/CD3D-antibody-EP4426-ab109531.html). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder zu melden, falls Sie weitere Fragen haben. Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-E292-ab32327.html). ab33524 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-PS1-ab33524.html). ab52959 Click here (or use the following: https://www.abcam.com/CD3-epsilon-antibody-EP449E-ab52959.html). ab109531 Click here (or use the following: https://www.abcam.com/CD3D-antibody-EP4426-ab109531.html). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zoegern Sie nicht, sich wioeder zu melden, falls Sie weitere Fragen haben.

    Read More

    Abcam Scientific Support

    Answered on Sep 22 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.