
Recombinant Anti-CD3 epsilon antibody [EP449E] (ab52959)
Rabbit recombinant monoclonal CD3 epsilon antibody [EP449E]. Validated in WB, IP, IHC and tested in Human. Cited in 10 publication(s). Independently reviewed in 1 review(s).
- Datasheet
- References (10)
- Protocols
Overview
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Product name
Anti-CD3 epsilon antibody [EP449E]
See all CD3 epsilon primary antibodies -
Description
Rabbit monoclonal [EP449E] to CD3 epsilon -
Host species
Rabbit -
Specificity
ab52959 reacts with CD3 epsilon. It does not cross-react with other subunits of CD3. -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CD3 epsilon aa 150-250. Derived from the cytoplasmic domain.
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Positive control
- Jurkat cell lysate and human spleen tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP449E -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab52959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). | |
IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
IP | 1/50. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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All lanes : Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/1000 dilution (purified)
Lane 1 : Jurkat( Human T cell leukemia T lymphocyte )membrane fraction cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) without membrane fraction cell lysate
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 20 secondsBlocking/Dilution buffer and concentration: 5% NFDM/TBST
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
ab52959 showing positive staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
ab52959 showing positive staining in Normal spleen tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
ab52959 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
ab52959 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)
ab52959 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Schaadt NS et al. Image analysis of immune cell patterns in the human mammary gland during the menstrual cycle refines lymphocytic lobulitis. Breast Cancer Res Treat N/A:N/A (2017). IHC-P ; Human . Read more (PubMed: 28444535) »
- Grabner A et al. Noninvasive Imaging of Acute Renal Allograft Rejection by Ultrasound Detection of Microbubbles Targeted to T-lymphocytes in Rats. Ultraschall Med 37:82-91 (2016). IHC-P . Read more (PubMed: 25919412) »