Name: Anti-CD3 epsilon antibody [EP449E]
Brand: RabMAb
SKU: ab52959
Rating: 5 (2 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP449E] to CD3 epsilon
Suitable for: WB, IHC-P, IP
Reacts with: Human

Carrier Free

Product name

Anti-CD3 epsilon antibody [EP449E]
See all CD3 epsilon primary antibodies
Description

Rabbit monoclonal [EP449E] to CD3 epsilon
Host species

Rabbit
Specificity

ab52959 reacts with CD3 epsilon. It does not cross-react with other subunits of CD3.
Tested applications

Suitable for: WB, IHC-P, IPmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide corresponding to Human CD3 epsilon aa 150-250. Derived from the cytoplasmic domain.
Database link: P07766
Positive control
- Jurkat cell lysate and human spleen tissue. IP: Jurkat whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP449E
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
- Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat membrane extract lysate (ab30130)
Recombinant Protein
- Recombinant human CD3 epsilon protein (Active) (ab167755)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P	(1)	1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP		1/20.

Notes
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/20.

Function

The CD3 complex mediates signal transduction.
Sequence similarities

Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
Cellular localization

Membrane.
Target information above from: UniProt accession P07766 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
- CD3e antigen epsilon polypeptide (TiT3 complex) antibody
- CD3E antigen epsilon polypeptide antibody
- CD3E antigen, epsilon subunit antibody
- CD3e molecule epsilon antibody
- CD3e molecule, epsilon (CD3 TCR complex) antibody
- CD3e molecule, epsilon (CD3-TCR complex) antibody
- CD3E_HUMAN antibody
- IMD18 antibody
- T cell antigen receptor complex epsilon subunit of T3 antibody
- T cell surface antigen T3/Leu 4 epsilon chain antibody
- T cell surface glycoprotein CD3 epsilon chain antibody
- T-cell surface antigen T3/Leu-4 epsilon chain antibody
- T-cell surface glycoprotein CD3 epsilon chain antibody
- T3E antibody
- TCRE antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-CD3 epsilon antibody [EP449E] (ab52959)

All lanes : Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/1000 dilution (purified)

Lane 1 : Jurkat( Human T cell leukemia T lymphocyte )membrane fraction cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) without membrane fraction cell lysate
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 20 seconds

Blocking/Dilution buffer and concentration: 5% NFDM/TBST
Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] (ab52959)

Purified ab52959 at 1/20 dilution (1µg) immunoprecipitating CD3 epsilon in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab52959 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52929 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing positive staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing positive staining in Normal spleen tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing negative staining in Normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (ab52959)

ab52959 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CD3 epsilon antibody [EP449E] (ab52959)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab52959? Please let us know so that we can cite the reference in this datasheet.

ab52959 has been referenced in 13 publications.

Schaadt NS et al. Graph-based description of tertiary lymphoid organs at single-cell level. PLoS Comput Biol 16:e1007385 (2020). PubMed: 32084130
Kim D et al. Somatic mTOR mutation in clonally expanded T lymphocytes associated with chronic graft versus host disease. Nat Commun 11:2246 (2020). PubMed: 32382059
Yang X et al. Misdiagnosis of primary pleural DLBCL as tuberculosis: A case report and literature review. Mol Clin Oncol 8:729-732 (2018). PubMed: 29732155
Schaadt NS et al. Image analysis of immune cell patterns in the human mammary gland during the menstrual cycle refines lymphocytic lobulitis. Breast Cancer Res Treat N/A:N/A (2017). IHC-P ; Human . PubMed: 28444535
Grabner A et al. Noninvasive Imaging of Acute Renal Allograft Rejection by Ultrasound Detection of Microbubbles Targeted to T-lymphocytes in Rats. Ultraschall Med 37:82-91 (2016). IHC-P . PubMed: 25919412

View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application

Western blot

Sample

Human Cell lysate - whole cell (T-cells)

Gel Running Conditions

Reduced Denaturing (4-20% Tris HCl)

Loading amount

7.69 µg

Specification

T-cells

Blocking step

Licor intercept TBS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 13 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (distal colon)

Specification

distal colon

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer

Permeabilization

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Isabel Romero Calvo

Verified customer

Submitted Jan 11 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (13)

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 epsilon antibody [EP449E]