Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (distal colon)
Specification
distal colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1/100
Secondary antibody
Name
Non-Abcam antibody was used: Dako REAL EnVision/HRP, Rabbit/Mouse (ENV)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Isabel Romero Calvo
Verified customer
Submitted Jan 11 2013