Recombinant Anti-CD3 epsilon antibody [EPR20752] (ab215212)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20752] to CD3 epsilon
- Suitable for: IHC-P, WB, IHC-Fr, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CD3 epsilon antibody [EPR20752]
See all CD3 epsilon primary antibodies -
Description
Rabbit monoclonal [EPR20752] to CD3 epsilon -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Fr, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse thymus and lymph node lysate; EL4.IL-2 whole cell lysate. IHC-P: Mouse spleen and thymus tissue. IHC-fr: Mouse spleen tissue. IP: EL4.IL-2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20752 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). |
|
WB |
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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IHC-Fr |
1/800.
Perform heat mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20) |
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IP |
1/30.
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Notes |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). |
WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
IHC-Fr
1/800. Perform heat mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20) |
IP
1/30. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12501 Mouse
- SwissProt: P22646 Mouse
- Unigene: 210361 Mouse
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilised mouse spleen tissue labelling CD3 epsilon with ab215212 at 1/800 dilution, foloowed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive membrane staining on cells localized in the periarterial lymphatic sheath in mouse spleen tissue section is observed (PMID: 20880086) Counterstained with DAPI.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. -
CD3 epsilon was immunoprecipitated from 10 μg of EL4.IL-2 (Mouse lymphoma T lymphocyte) whole cell lysate with ab215212 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215212 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution
Lane 1: EL4.IL-2 (Mouse lymphoma T lymphocyte) whole cell lysate 10 μg (input).
Lane 2: ab215212 IP in EL4.IL-2 whole cell lysate (+)
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215212 in EL4.IL-2 whole cell lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes -
All lanes : Anti-CD3 epsilon antibody [EPR20752] (ab215212) at 1/1000 dilution
Lanes 1-2 : Mouse lymph node
Lane 3 : EL4.IL-2 (mouse lymphoma T lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 21 kDa
Observed band size: 21 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lanes 1-2: 3 minutes
Lane 3: 15 seconds -
Immunohistochemical analysis of Paraffin-embedded sections mouse thymus tissue labeling CD3 epsilon with ab215212 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Mainly membranous staining on T cells in mouse thymus is observed (PMID: 18648532). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Paraffin-embedded sections mouse spleen tissue labeling CD3 epsilon with ab215212 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Mainly membranous staining on T cells in mouse spleen is observed (PMID: 18648532). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab215212 has been referenced in 4 publications.
- Pierini S et al. Combination of vasculature targeting, hypofractionated radiotherapy, and immune checkpoint inhibitor elicits potent antitumor immune response and blocks tumor progression. J Immunother Cancer 9:N/A (2021). PubMed: 33563772
- Gao L et al. Kinsenoside Alleviates Alcoholic Liver Injury by Reducing Oxidative Stress, Inhibiting Endoplasmic Reticulum Stress, and Regulating AMPK-Dependent Autophagy. Front Pharmacol 12:747325 (2021). PubMed: 35115920
- Wheeler MA et al. MAFG-driven astrocytes promote CNS inflammation. Nature 578:593-599 (2020). PubMed: 32051591
- Zhang SR et al. IL-37 increases in patients after ischemic stroke and protects from inflammatory brain injury, motor impairment and lung infection in mice. Sci Rep 9:6922 (2019). PubMed: 31061403