Recombinant
RabMAb

Recombinant Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (ab231830)

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Overview

  • Product name

    Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free
    See all CD3 epsilon primary antibodies

  • Description

    Rabbit monoclonal [EPR20752] to CD3 epsilon - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IHC-Fr, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide within Mouse CD3 epsilon aa 1-100. The exact sequence is proprietary.
    Database link: P22646

  • Positive control

    • IHC-P: Mouse spleen tissue.

  • General notes

    Ab231830 is the carrier-free version of ab215212. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab231830 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab231830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
WB Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.
IHC-Fr Use at an assay dependent concentration.

Perform heat mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)
IP Use at an assay dependent concentration.

Target

  • Function

    The CD3 complex mediates signal transduction.

  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (ab231830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (ab231830)

      Immunohistochemical analysis of Paraffin-embedded sections mouse thymus tissue labeling CD3 epsilon with ab215212 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP)  ready to use. Mainly membranous staining on T cells in mouse thymus is observed (PMID: 18648532). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215212).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (ab231830)
      Immunohistochemistry (Frozen sections) - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (ab231830)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilised mouse spleen tissue labelling CD3 epsilon with ab215212 at 1/800 dilution, foloowed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive membrane staining on cells localized in the periarterial lymphatic sheath in mouse spleen tissue section is observed (PMID: 20880086) Counterstained with DAPI.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215212).

    • Immunoprecipitation - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (ab231830)
      Immunoprecipitation - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (ab231830)

      CD3 epsilon was immunoprecipitated from 10 μg of EL4.IL-2 (Mouse lymphoma T lymphocyte) whole cell lysate with ab215212 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215212 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
      Lane 1: EL4.IL-2 (Mouse lymphoma T lymphocyte) whole cell lysate 10 μg (input).
      Lane 2: ab215212 IP in EL4.IL-2 whole cell lysate (+)
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215212 in EL4.IL-2 whole cell lysate (-).
      Blocking and dilution buffer and concentration: 5% NFDM/TBST
      Exposure time: 3 minutes

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215212).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (ab231830)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (ab231830)

      Immunohistochemical analysis of Paraffin-embedded sections mouse spleen tissue labeling CD3 epsilon with ab215212 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP)  ready to use. Mainly membranous staining on T cells in mouse spleen is observed (PMID: 18648532). Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215212).

      Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    ab231830 has not yet been referenced specifically in any publications.

    Publishing research using ab231830? Please let us know so that we can cite the reference in this datasheet.

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