RecombinantRabMAb

Recombinant Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)

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Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
  • Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
  • Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22667-12] to CD3 epsilon - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free
    See all CD3 epsilon primary antibodies

  • Description

    Rabbit monoclonal [EPR22667-12] to CD3 epsilon - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment within Mouse CD3 epsilon aa 1-100. The exact sequence is proprietary.
    Database link: P22646

  • Positive control

    • IP: EL4.IL2 whole cell lysate. IHC-P: Mouse spleen and lung tissue. ICC/IF: EL4.IL2 cells.

  • General notes

    Ab255697 is the carrier-free version of ab231775. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab255697 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab255697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 18, 22 kDa (predicted molecular weight: 21 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      The CD3 complex mediates signal transduction.

    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD3 epsilon antibody
        • CD3e antibody
        • CD3e antigen antibody
        • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
        • CD3E antigen epsilon polypeptide antibody
        • CD3E antigen, epsilon subunit antibody
        • CD3e molecule epsilon antibody
        • CD3e molecule, epsilon (CD3 TCR complex) antibody
        • CD3e molecule, epsilon (CD3-TCR complex) antibody
        • CD3E_HUMAN antibody
        • IMD18 antibody
        • T cell antigen receptor complex epsilon subunit of T3 antibody
        • T cell surface antigen T3/Leu 4 epsilon chain antibody
        • T cell surface glycoprotein CD3 epsilon chain antibody
        • T-cell surface antigen T3/Leu-4 epsilon chain antibody
        • T-cell surface glycoprotein CD3 epsilon chain antibody
        • T3E antibody
        • TCRE antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
        Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL2 (mouse lymphoma T lymphocyte cell line) cells labeling CD3 epsilon with ab231775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in EL4.IL2 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

        Antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231775).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3 epsilon with ab231775 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the mouse spleen (PMID: 18648532) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

        Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231775).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)

        Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD3 epsilon with ab231775 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the T lymphocytes on mouse lung (PMID: 22888136) is observed. Counter stained with hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

        Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231775).

      • Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
        Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)

        CD3 epsilon was immunoprecipitated from 0.35 mg of EL4.IL2 (mouse lymphoma T lymphocyte cell line) whole cell lysate with ab231775 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab231775 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

        Lane 1: EL4.IL2 whole cell lysate [amount of lysate 10 μg (Input).
        Lane 2: ab231775 IP in EL4.IL2 whole cell lysate.
        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab231775 in EL4.IL2 whole cell lysate.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.
        Exposure time: 30 seconds.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231775).

      • Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
        Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab255697? Please let us know so that we can cite the reference in this datasheet.

      ab255697 has not yet been referenced specifically in any publications.

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