Recombinant Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)
![Immunocytochemistry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)](https://www.abcam.com/ps/products/133/ab133628/Images/ab133628-479419-AntiCD3epsilonantibodyICCab133628.jpg "Immunocytochemistry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5361(2)] to CD3 epsilon
- Suitable for: WB, IP, Flow Cyt, ICC
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD3 epsilon antibody [EPR5361(2)]
See all CD3 epsilon primary antibodies -
Description
Rabbit monoclonal [EPR5361(2)] to CD3 epsilon -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cyt, ICCmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat and Human thymus lysates, HuT78 cells IP: Hut-78 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5361(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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| IP |
1/10 - 1/100.
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| Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC |
Use at an assay dependent concentration.
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| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
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IP
1/10 - 1/100. |
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Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC
Use at an assay dependent concentration. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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Immunocytochemistry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed Jurkat (human T cell leukemia T lymphocyte) cells labelling CD3 epsilon with ab133628 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cells. Counterstained with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1:200 dilution.
The negative controls was Raji cell line. -
![Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)](https://www.abcam.com/ps/products/133/ab133628/Images/ab133628-479939-AntiCD3epsilonantibodyflow2.jpg)
Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab133628 at 1/100 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)](https://www.abcam.com/ps/products/133/ab133628/Images/ab133628-479564-ab133628147519anticd3epsilonantibodyepr53612flowcytometryupdate.jpg)
Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)Flow cytometry overlay histogram of Jurkat (Human T cell leukemia T lymphocyte) cells labelling CD3 epsilon with ab133628 at 1/100 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)](https://www.abcam.com/ps/products/133/ab133628/Images/ab133628-479424-ab133628-AntiCD3-epsilon-human-tissue-and-cell-002.jpg)
Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)All lanes : Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628) at 1/1000 dilution (Anti-CD3 epsilon antibody)
Lane 1 : Human peripheral blood mononuclear cell (PBMC) lysate with NFDM/TBST
Lane 2 : Human thymus tissue lysate with NFDM/TBST
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 10 seconds -
![Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)](https://www.abcam.com/ps/products/133/ab133628/Images/ab133628-5-anti-cd3-epsilon-antibody-epr5361-2-immunoprecipitation-hut78-human.jpg)
Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)Purified ab133628 at 1/50 dilution (2µg) immunoprecipitating CD3 epsilon in Hut-78 whole cell lysate.
Lane 1 (input): Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab133628 + Hut-78 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133628 in Hut-78 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDa -
![Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)](https://www.abcam.com/ps/products/133/ab133628/Images/ab133628-3-benefits-of-recombinant-antibodies.png)
Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133628 has been referenced in 1 publication.
- Li L et al. Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling. Proc Natl Acad Sci U S A 114:E5891-E5899 (2017). PubMed: 28659468