Recombinant Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CD3 epsilon antibody [EPR5361(2)]
See all CD3 epsilon primary antibodies -
Description
Rabbit monoclonal [EPR5361(2)] to CD3 epsilon -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues in the extracellular domain of Human CD3 epsilon (UniProt ID: P07766).
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Positive control
- Jurkat and Human thymus lysates, HuT78 cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5361(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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![Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)](https://www.abcam.com/ps/products/133/ab133628/Images/ab133628-147520-anti-cd3-epsilon-antibody-epr53612-western-blot.jpg)
Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)All lanes : Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human thymus cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa -
![Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)](https://www.abcam.com/ps/products/133/ab133628/Images/ab133628-147519-anti-cd3-epsilon-antibody-epr53612-flow-cytometry.jpg)
Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)Flow cytometrical analysis of CD3 epsilon in HuT78 cells stained with ab133628 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
References
This product has been referenced in:
- Li L et al. Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling. Proc Natl Acad Sci U S A 114:E5891-E5899 (2017). Read more (PubMed: 28659468) »
