Key features and details
- Mouse monoclonal [MM0167-3F43] to CD3 epsilon
- Suitable for: IHC-P, IHC-Fr, Flow Cyt, Cellular Activation
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD3 epsilon antibody [MM0167-3F43]
See all CD3 epsilon primary antibodies
DescriptionMouse monoclonal [MM0167-3F43] to CD3 epsilon
Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cyt, Cellular Activationmore details
Species reactivityReacts with: Human
Human recombinant CD3 epsilon
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Purification notesThe IgG fraction of culture supernatant was purified by Protein G affinity chromatography.
Our Abpromise guarantee covers the use of ab89205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/50 - 1/100.|
|Flow Cyt||1/50 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Cellular Activation||Use at an assay dependent concentration.|
FunctionThe CD3 complex mediates signal transduction.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
- Information by UniProt
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CD3 with ab89205. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
ab89205 has not yet been referenced specifically in any publications.