Anti-CD3 epsilon antibody [OKT3] (Allophycocyanin) (ab210315)

Overview

  • Product name

    Anti-CD3 epsilon antibody [OKT3] (Allophycocyanin)
    See all CD3 epsilon primary antibodies
  • Description

    Mouse monoclonal [OKT3] to CD3 epsilon (Allophycocyanin)
  • Host species

    Mouse
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Specificity

    This antibody reacts with CD3 epsilon
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Positive control

    • Human peripheral blood lymphocyte
  • General notes

    This antibody is a widely used phenotypic marker for human T cells. In addition, as the CD3 epsilon subunit of the TCR complex contains intracellular signaling domains, binding of ab210315 to CD3 epsilon can induce cell activation. 

Properties

Applications

Our Abpromise guarantee covers the use of ab210315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 105-8 cells.

in 100 μl volume.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Images

  • Flow cytometry analysis of Human peripheral blood lymphocyte labeling CD3 epsilon using 5 μl (0.125 μg) of ab210315 (solid line) and 0.125 μg of Allophycocyanin Mouse IgG2a control (dashed line).

References

ab210315 has not yet been referenced specifically in any publications.

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