Anti-CD3 epsilon antibody [OKT3] - Low endotoxin, Azide free (ab210317)
Key features and details
- Mouse monoclonal [OKT3] to CD3 epsilon - Low endotoxin, Azide free
- Suitable for: IHC-Fr, IHC-P, Functional Studies, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-CD3 epsilon antibody [OKT3] - Low endotoxin, Azide free
See all CD3 epsilon primary antibodies -
Description
Mouse monoclonal [OKT3] to CD3 epsilon - Low endotoxin, Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IHC-P, Functional Studies, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Tissue, cells or virus corresponding to CD3 epsilon. Sheep Erythrocyte Rosette-purified Human T Cells
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General notes
ab210317 is a widely used phenotypic marker for human T cells. In addition, as the CD3 epsilon subunit of the TCR complex contains intracellular signaling domains, binding of ab210317 to CD3 epsilon can induce cell activation.
Endotoxin Level: Less than or equal to 0.01 EU/ug, as determined by the LaL assay.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Constituents: 0.87% Sodium chloride, 0.16% Monobasic dihydrogen sodium phosphate -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from tissue culture supernatant. >90% pure as determined by SDS-PAGE analysis -
Clonality
Monoclonal -
Clone number
OKT3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| IHC-P |
Use at an assay dependent concentration.
|
|
| Functional Studies |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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Functional Studies
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 742330 Chimpanzee
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab210317 has not yet been referenced specifically in any publications.
