Overview

  • Product name
    Anti-CD3 epsilon antibody [PS1]
    See all CD3 epsilon primary antibodies
  • Description
    Mouse monoclonal [PS1] to CD3 epsilon
  • Host species
    Mouse
  • Specificity
    ab33524 recognises the e-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta and eta) with MW ranging from 16-28kDa. The clone number has been updated from (0.N.47) to (PS1) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Recombinant non glycosylated epsilon chain of CD3 protein.

  • Positive control
    • Tonsils

Properties

Applications

Our Abpromise guarantee covers the use of ab33524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
  • Application notes
    IHC-P: 1/30 - 1/60. This antibody stains normal and neoplastic T Cells. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6 for 10-20 min., followed by cooling at room temperature for 20min.
    IHC-Fr: 1/30 - 1/60.
    WB: 1/100. Predicted molecular weight: 23 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      The CD3 complex mediates signal transduction.
    • Sequence similarities
      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD3 epsilon antibody
      • CD3e antibody
      • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3E antigen, epsilon subunit antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule, epsilon (CD3 TCR complex) antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3E_HUMAN antibody
      • IMD18 antibody
      • T cell antigen receptor complex epsilon subunit of T3 antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T3E antibody
      • TCRE antibody
      see all

    References

    ab33524 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Question
    Answer

    Thank you for contacting us. I apologise for the inconveniences.

    Unfortunately, ab33524 is an unpurified antibody, it is provided as Tissue culture supernatant. We can only provide concentrations for purified antibodies as unpurified antibody preparations vary significantly in specific antibody concentration.

    Nevertheless, I recommend you having a look at our website FAQs, were you could find a guideline for concentration estimation, depending on the application you are using the antibody for. You will find this valuable information at:

    https://www.abcam.com/index.html?pageconfig=technicalfaqs#8

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question
    Answer

    Vielen dank fuer Ihren Anruf. Ich habe nun ein paar Antikoerper fuer Sie herrausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und fuer IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblaetern mit mehr Informatinen zugelangen. ab32327 Vielen Dank für Ihren Anruf. Ich habe nun ein paar Antikörper für Sie herausgesucht, die nur humane T Zellen erkennen und nicht T Zellen aus der Ratte und für IHC-P getestet und garantiert sind. Bitte folgen Sie den jeweiligen Links um zu den Datenblättern mit mehr Informationen zugelangen. ab32327 Click here (or use the following: https://www.abcam.com/index.html?datasheet=32327). ab33524 Click here (or use the following: https://www.abcam.com/index.html?datasheet=33524). ab52959 Click here (or use the following: https://www.abcam.com/index.html?datasheet=52959). ab109531 Click here (or use the following: https://www.abcam.com/index.html?datasheet=109531). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder zu melden, falls Sie weitere Fragen haben. Click here (or use the following: https://www.abcam.com/index.html?datasheet=32327). ab33524 Click here (or use the following: https://www.abcam.com/index.html?datasheet=33524). ab52959 Click here (or use the following: https://www.abcam.com/index.html?datasheet=52959). ab109531 Click here (or use the following: https://www.abcam.com/index.html?datasheet=109531). Ich hoffe, diese Liste hilft Ihnen weiter. Bitte zoegern Sie nicht, sich wioeder zu melden, falls Sie weitere Fragen haben.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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