Key features and details
- Rabbit polyclonal to CD3 zeta
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD3 zeta antibody
See all CD3 zeta primary antibodies
DescriptionRabbit polyclonal to CD3 zeta
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Guinea pig, Cow, Cat, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human CD3 zeta aa 114-164. The exact sequence is proprietary.
Database link: P20963
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226263 was affinity purified using an epitope specific to CD3 zeta immobilized on solid support.
Our Abpromise guarantee covers the use of ab226263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 19 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionProbable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Involvement in diseaseDefects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
Sequence similaritiesBelongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains.
DomainThe ITAM domains mediate interaction with SHB.
modificationsPhosphorylated on Tyr residues after T-cell receptor triggering.
- Information by UniProt
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
All lanes : Anti-CD3 zeta antibody (ab226263) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 10 seconds
CD3 zeta was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226263 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226263 at 1 µg/ml.
Lane 1: ab226263 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
Cells prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226263 has not yet been referenced specifically in any publications.