Recombinant Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD3 zeta antibody [BL-336-1B2]
See all CD3 zeta primary antibodies -
Description
Rabbit monoclonal [BL-336-1B2] to CD3 zeta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CD3 zeta aa 150-164 (C terminal). The exact sequence is proprietary. NP_932170.1 and Gene ID 919.
Database link: P20963 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-336-1B2 -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab243874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at an assay dependent concentration. Use 20µl/mg lysate. |
Target
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Function
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. -
Involvement in disease
Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens. -
Sequence similarities
Belongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains. -
Domain
The ITAM domains mediate interaction with SHB. -
Post-translational
modificationsPhosphorylated on Tyr residues after T-cell receptor triggering. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 919 Human
- Omim: 186780 Human
- SwissProt: P20963 Human
- Unigene: 156445 Human
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Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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![Immunoprecipitation - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)](https://www.abcam.com/ps/products/243/ab243874/Images/ab243874-334286-anti-cd3-zeta-antibody-bl-336-1b2-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)CD3 zeta was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243874 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243874 at 1/1000 dilution.Lane 1: ab243874 IP in Jurkat whole cell lysate.
Lane 2: Contol IgG in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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![Western blot - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)](https://www.abcam.com/ps/products/243/ab243874/Images/ab243874-334285-anti-cd3-zeta-antibody-bl-336-1b2-western-blot.jpg)
Western blot - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)Western blot analysis using ab243874 at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate (50 µg).
Lane 2: MOLT-4 whole cell lysate (50 µg).
Lane 3: K-562 whole cell lysate (50 µg).
Lane 4: SK whole cell lysate (50 µg).
Lane 5: HeLa whole cell lysate (50 µg).
Lane 6: HEK-293Y whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)](https://www.abcam.com/ps/products/243/ab243874/Images/ab243874-334284-anti-cd3-zeta-antibody-bl-336-1b2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3 zeta using ab243874 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)](https://www.abcam.com/ps/products/243/ab243874/Images/ab243874-334283-anti-cd3-zeta-antibody-bl-336-1b2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] (ab243874)Formalin-fixed, paraffin-embedded human metastatic lymph node tissue stained for CD3 zeta using ab243874 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
References
ab243874 has not yet been referenced specifically in any publications.
