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Immunology Adaptive Immunity T Cells CD
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Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827)

  • Datasheet
  • SDS
Reviews (6)Q&A (1)References (6)

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Key features and details

  • Armenian hamster monoclonal [H146-968] to CD3 zeta - BSA and Azide free
  • Reacts with: Mouse
  • Isotype: IgG

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Protein
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Recombinant Human CD3 zeta protein (ab139254)

View more associated products

Overview

  • Product name

    Anti-CD3 zeta antibody [H146-968] - BSA and Azide free
    See all CD3 zeta primary antibodies
  • Description

    Armenian hamster monoclonal [H146-968] to CD3 zeta - BSA and Azide free
  • Host species

    Armenian hamster
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Chicken, Human, Pig
  • Immunogen

    Synthetic peptide:

    DTYDALHMQTLAPR

    , corresponding to amino acids 151-164 of Mouse CD3 zeta.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab119827 is purified from cell culture supernatant by protein A affinity chromatography and 0.2 µm filter sterilized. Purity is > 95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    H146-968
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD

Associated products

  • Alternative Versions

    • PE Anti-CD3 zeta antibody [H146-968] (ab243075)
    • FITC Anti-CD3 zeta antibody [H146-968] (ab91493)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Armenian Hamster IgG monoclonal [HTK888] - Isotype Control (ab18479)
  • Recombinant Protein

    • Recombinant Human CD3 zeta protein (ab139254)

Target

  • Function

    Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
  • Involvement in disease

    Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
  • Sequence similarities

    Belongs to the CD3Z/FCER1G family.
    Contains 3 ITAM domains.
  • Domain

    The ITAM domains mediate interaction with SHB.
  • Post-translational
    modifications

    Phosphorylated on Tyr residues after T-cell receptor triggering.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P20963 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395911 Chicken
    • Entrez Gene: 919 Human
    • Entrez Gene: 12503 Mouse
    • Entrez Gene: 397302 Pig
    • Entrez Gene: 100008617 Rabbit
    • Entrez Gene: 25300 Rat
    • Omim: 186780 Human
    • SwissProt: P20963 Human
    • SwissProt: P24161 Mouse
    • SwissProt: Q9XSJ9 Pig
    • SwissProt: Q9TUF8 Rabbit
    • Unigene: 156445 Human
    • Unigene: 217308 Mouse
    • Unigene: 475205 Mouse
    • Unigene: 9997 Rat
    see all
  • Alternative names

    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • CD247 antibody
    • CD247 antigen antibody
    • Cd247 molecule antibody
    • Cd3 antibody
    • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
    • CD3 antigen, zeta subunit antibody
    • CD3-eta antibody
    • CD3H antibody
    • CD3Q antibody
    • Cd3z antibody
    • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
    • CD3Z_HUMAN antibody
    • CD3zeta antibody
    • CD3zeta chain antibody
    • MGC140430 antibody
    • T cell receptor T3 zeta chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 zeta chain antibody
    • T-cell surface glycoprotein CD3 zeta chain antibody
    • T3z antibody
    • TCR zeta chain antibody
    • TCRk antibody
    • TCRZ antibody
    • TCRzeta antibody
    see all

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab119827? Please let us know so that we can cite the reference in this datasheet.

ab119827 has been referenced in 6 publications.

  • Wei J  et al. PD-1 silencing impairs the anti-tumor function of chimeric antigen receptor modified T cells by inhibiting proliferation activity. J Immunother Cancer 7:209 (2019). PubMed: 31391096
  • Jelencic V  et al. NK cell receptor NKG2D sets activation threshold for the NCR1 receptor early in NK cell development. Nat Immunol 19:1083-1092 (2018). PubMed: 30224819
  • Iida T  et al. Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4-containing lysosomes upon activation of CD4+ T cells. J Immunol 165:5062-8 (2000). PubMed: 11046036
  • Kosugi A  et al. Translocation of tyrosine-phosphorylated TCRzeta chain to glycolipid-enriched membrane domains upon T cell activation. Int Immunol 11:1395-401 (1999). PubMed: 10464160
  • Bäckström BT  et al. Signaling efficiency of the T cell receptor controlled by a single amino acid in the beta chain constant region. J Exp Med 186:1933-8 (1997). PubMed: 9382891
  • Rozdzial MM  et al. Developmental regulation of the TCR zeta-chain. Differential expression and tyrosine phosphorylation of the TCR zeta-chain in resting immature and mature T lymphocytes. J Immunol 153:1563-80 (1994). PubMed: 7519206

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Immunocytochemistry abreview for Anti-CD3 zeta antibody [H146-968] - BSA and Azide free

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cell (Peritoneal aspirate smear)
Permeabilization
No
Specification
Peritoneal aspirate smear
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Oct 20 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 zeta antibody [H146-968] - BSA and Azide free

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Mar 17 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 zeta antibody [H146-968] - BSA and Azide free

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Mar 17 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 zeta antibody [H146-968] - BSA and Azide free

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Mar 17 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 zeta antibody [H146-968] - BSA and Azide free

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Mar 17 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 zeta antibody [H146-968] - BSA and Azide free

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Armenian hamster Tissue sections (mouse small and large intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieving Buffer pH6
Permeabilization
No
Specification
mouse small and large intestine
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 20% · Temperature: 37°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 07 2018

Question

The samples are of Human origin.

Regards,

Read More

Abcam community

Verified customer

Asked on Dec 13 2012

Answer

Thank you for your reply.

Unfortunately, I am sorry to confirm that I have checked the CD3 zeta antibodies that have been tested in flow cytometry and human samples. These have epitopes in the cytoplasmic domain, and so would require permeabilization, so intracellular as well as membrane CD3 zeta would be detected.

We are happy to be able to offer custom antibody services through Epitomics, an Abcam company. I hope this may be a useful? You may like to consider discussing your epitope requirements with them to find out if they can produce something suitable. For more information about these services visit:

http://www.epitomics.com/services/
email: service@epitomics.com

Alternatively, please contact Li Li at Epitomics:

li.li@epitomics.com

I am sorry we do not have a suitable product to suggest on this occasion, I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions.

Read More

Abcam Scientific Support

Answered on Dec 13 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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