Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-CD3 zeta antibody [H146-968] - BSA and Azide free
See all CD3 zeta primary antibodies -
DescriptionArmenian hamster monoclonal [H146-968] to CD3 zeta - BSA and Azide free
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Host speciesArmenian hamster
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Tested applicationsSuitable for: WB, IP, ICC, Flow Cytmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Pig
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Immunogen
Synthetic peptide:
DTYDALHMQTLAPR
, corresponding to amino acids 151-164 of Mouse CD3 zeta. -
Positive control
- Mouse splenocyte lysate.
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General notes
This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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Purification notesab119827 is purified from cell culture supernatant by protein A affinity chromatography and 0.2 µm filter sterilized. Purity is > 95% by SDS-PAGE.
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ClonalityMonoclonal
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Clone numberH146-968
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab119827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. | |
| IP | Use at an assay dependent concentration. | |
| ICC | Use a concentration of 4 µg/ml. | |
| Flow Cyt | Use at an assay dependent concentration. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionProbable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
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Involvement in diseaseDefects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
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Sequence similaritiesBelongs to the CD3Z/FCER1G family.
Contains 3 ITAM domains. -
DomainThe ITAM domains mediate interaction with SHB.
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Post-translational
modificationsPhosphorylated on Tyr residues after T-cell receptor triggering. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 395911 Chicken
- Entrez Gene: 919 Human
- Entrez Gene: 12503 Mouse
- Entrez Gene: 397302 Pig
- Entrez Gene: 100008617 Rabbit
- Entrez Gene: 25300 Rat
- Omim: 186780 Human
- SwissProt: P20963 Human
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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![Western blot - Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827)](https://www.abcam.com/ps/products/119/ab119827/Images/CD3-zeta-Primary-antibodies-ab119827-1.bmp)
Western blot - Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827)Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827) at 1 µg/ml + Mouse splenocyte lysate
Performed under reducing conditions.
Predicted band size: 19 kDa
Protocols
References
This product has been referenced in:
- Iida T et al. Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4-containing lysosomes upon activation of CD4+ T cells. J Immunol 165:5062-8 (2000). Read more (PubMed: 11046036) »
- Kosugi A et al. Translocation of tyrosine-phosphorylated TCRzeta chain to glycolipid-enriched membrane domains upon T cell activation. Int Immunol 11:1395-401 (1999). Read more (PubMed: 10464160) »
