Anti-CD3 zeta antibody [H146-968] (FITC) (ab91493)

Overview

  • Product name
    Anti-CD3 zeta antibody [H146-968] (FITC)
    See all CD3 zeta primary antibodies
  • Description
    Armenian hamster monoclonal [H146-968] to CD3 zeta (FITC)
  • Host species
    Armenian hamster
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Cow, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    DTYDALHMQALPPR

    , corresponding to amino acids 151-164 of Mouse CD3 zeta

  • General notes

    Purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 8
    Preservative: 0.097% Sodium azide
    Constituent: Tris buffered saline
  • Concentration information loading...
  • Purity
    Size exclusion
  • Purification notes
    Purified from TCS
  • Clonality
    Monoclonal
  • Clone number
    H146-968
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use at a concentration of 2 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
    • Involvement in disease
      Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.
    • Sequence similarities
      Belongs to the CD3Z/FCER1G family.
      Contains 3 ITAM domains.
    • Domain
      The ITAM domains mediate interaction with SHB.
    • Post-translational
      modifications
      Phosphorylated on Tyr residues after T-cell receptor triggering.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • CD247 antibody
      • CD247 antigen antibody
      • Cd247 molecule antibody
      • Cd3 antibody
      • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
      • CD3 antigen, zeta subunit antibody
      • CD3-eta antibody
      • CD3H antibody
      • CD3Q antibody
      • Cd3z antibody
      • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
      • CD3Z_HUMAN antibody
      • CD3zeta antibody
      • CD3zeta chain antibody
      • MGC140430 antibody
      • T cell receptor T3 zeta chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 zeta chain antibody
      • T-cell surface glycoprotein CD3 zeta chain antibody
      • T3z antibody
      • TCR zeta chain antibody
      • TCRk antibody
      • TCRZ antibody
      • TCRzeta antibody
      see all

    References

    ab91493 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (colon adenocarcinoma)
    Permeabilization
    Yes - 0.2% TritonX in 1XPBS
    Specification
    colon adenocarcinoma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 500µg/mL · Temperature: 22°C
    Fixative
    fresh tissue

    Abcam user community

    Verified customer

    Submitted Feb 04 2019

    Application
    Flow Cytometry
    Sample
    Chicken Cell (PBMC)
    Permeabilization
    Yes - BD Fix/Perm
    Gating Strategy
    Lymphocytes/Singlets/CD3/CD3zeta
    Specification
    PBMC
    Preparation
    Cell harvesting/tissue preparation method: Ficoll
    Sample buffer: PBS/5% FCS
    Fixation
    BD Fix/Perm

    Stig Henrik Andersen

    Verified customer

    Submitted May 19 2017

    Answer

    We do not have any more of ab11505 in stock. Are you able to use the PE-conjugated version of the same clone, ab33395, or the unconjugated version, ab11218? Alternatively, we have a FITC-conjugated hamster monoclonal antibody that is raised against mouse CD3 zeta that will recognize total CD3 zeta, phosphorylated and non-phosphorylated: ab91493. These have all been tested in flow cytometry (and for the unconjugated antibody, several other applications).

    Read More

    Question
    Answer

    Thank you for your reply.

    Unfortunately, I am sorry to confirm that I have checked the CD3 zeta antibodies that have been tested in flow cytometry and human samples. These have epitopes in the cytoplasmic domain, and so would require permeabilization, so intracellular as well as membrane CD3 zeta would be detected.

    We are happy to be able to offer custom antibody services through Epitomics, an Abcam company. I hope this may be a useful? You may like to consider discussing your epitope requirements with them to find out if they can produce something suitable. For more information about these services visit:

    http://www.epitomics.com/services/
    email: service@epitomics.com

    Alternatively, please contact Li Li at Epitomics:

    li.li@epitomics.com

    I am sorry we do not have a suitable product to suggest on this occasion, I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions.

    Read More

    Answer

    Thank you for your enquiry.

    I have done some literature searching and am not able to find any information to indicate that the membrane and cytoplasmic forms of CD3 Zeta are different, apart from the localization. Therefore, I would suggest the CD3 zeta antibodies we have will detect both. I am sorry it appears it would not be possible to distinguish between the two unless you observe the cellular localization of the staining in IHC-P, IHC-Fr or ICC-IF.

    The information I have found includes the following: 'CD3 is initially expressed in the cytoplasm of pro-thymocytes, the stem cells from which T-cells arise in the thymus. The pro-thymocytes differentiate into common thymocytes, and then into medullary thymocytes, and it is at this latter stage that CD3 antigen begins to migrate to the cell membrane'

    If you do have any further information on any differences between the CD3 zeta protein found at these two cellular localizations, please do not hesitate to let me know. I will be happy to review the immunogens for the antibodies if there are any more details.

    Good luck with your research.

    Read More

    Answer

    Thank you for your response.

    ab97077 is a goat anti-rabbit secondary so it is unlikely to react with ab91493 (host species hamster). However, ab97037 is goat anti-mouse secondary antibody and it may cross-react with hamster IgG since mouse and hamster are relatively close species taxonomically.

    Therefore, it may be worth checking any potential cross-reactivity in a separate experiment ie. ab91493 with ab97037.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Answer

    Thank you for your enquiry.

    Could you please confirm if the customer would like to perform double or triple staining? I am a bit confused why the two selected secondary antibodies ie ab97037 and ab97077 (Cy5 ®) have the same fluorophore? Would it be better to select three secondary antibodies with three different conjugates? Could you please explain?

    I look forward to hearing from you soon.

    Read More

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