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Dear Tech support
One of our customers wishes to perform a Flow Cytometry procedure in which he will use antibodies ab97037,ab97077 as secondaries. Above secondaries will be used with a Rabbit and Mouse primary antibodies.
Another primary to be used is ab91493 - Would the anti Rabbit and anti Mouse secondaries cross react with aPrimary antibody made in Hamster?
Thank you very much for your kind support.
Asked on Nov 12 2012
Thank you for your enquiry.
Could you please confirm if the customer would like to perform double or triple staining? I am a bit confused why the two selected secondary antibodies ie ab97037 and ab97077 (Cy5 ®) have the same fluorophore? Would it be better to select three secondary antibodies with three different conjugates? Could you please explain?
I look forward to hearing from you soon.
Answered on Nov 12 2012