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The customer plans to do two flow cytometry runs as follows:
1. ab91493 / ab78366+ab97037
2.ab91493 / ab86141+ab97077
Do you think the secondary antibodies might react with ab91493?
It is a good idea to allow for all three antibodies to be tested on one run, given ab91493 presence what would be a right flurophores choice for the secondary antibodies against ab78366, ab86141?
Thank you very much for the assistance.
Asked on Nov 13 2012
Thank you for your response.
ab97077 is a goat anti-rabbit secondary so it is unlikely to react with ab91493 (host species hamster). However, ab97037 is goat anti-mouse secondary antibody and it may cross-react with hamster IgG since mouse and hamster are relatively close species taxonomically.
Therefore, it may be worth checking any potential cross-reactivity in a separate experiment ie. ab91493 with ab97037.
If you need any further assistance in the future, please do not hesitate to contact me.
Answered on Nov 13 2012