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Dear Tech support
I'm looking for an antibody against cytoplasmic or intracellular form of CD3 Zeta. So far I was only able to find antibodies against it's membrane form, could you assist with finding the appropriate antibody?
Thank you for your kind help.
Asked on Nov 13 2012
Thank you for your enquiry.
I have done some literature searching and am not able to find any information to indicate that the membrane and cytoplasmic forms of CD3 Zeta are different, apart from the localization. Therefore, I would suggest the CD3 zeta antibodies we have will detect both. I am sorry it appears it would not be possible to distinguish between the two unless you observe the cellular localization of the staining in IHC-P, IHC-Fr or ICC-IF.
The information I have found includes the following: 'CD3 is initially expressed in the cytoplasm of pro-thymocytes, the stem cells from which T-cells arise in the thymus. The pro-thymocytes differentiate into common thymocytes, and then into medullary thymocytes, and it is at this latter stage that CD3 antigen begins to migrate to the cell membrane'
If you do have any further information on any differences between the CD3 zeta protein found at these two cellular localizations, please do not hesitate to let me know. I will be happy to review the immunogens for the antibodies if there are any more details.
Good luck with your research.
Answered on Nov 13 2012