Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein L purified
Our Abpromise guarantee covers the use of ab26864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 46 kDa (predicted molecular weight: 64 kDa).|
FunctionReceptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
Sequence similaritiesContains 6 TNFR-Cys repeats.
modificationsPhosphorylated on serine and tyrosine residues.
Cellular localizationCytoplasm and Cell membrane.
- Information by UniProt
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
Anti-CD30 antibody (ab26864) at 1/2000 dilution + human CD30 fusion protein (amino acids 171-226 )
HRP conjugated goat anti IgY/Fc at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 64 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Block membrane with 5% non-fat milk in PBS-T for 1 hour at room temperature or longer at 4°C
Incubate membrane with ab26864 at 1/2000 dilution with 1% milk in PBS-T at RT for 1 hour
Rinse 3 times with PBS-T, then wash membrane 3 times for 5 mins with PBS-T
Incubate with HRP-conjugated goat anti IgY/Fc at 1/1000 dilution for with 1% milk PBS-T at RT for 1 hour
Rinse 3 times with PBS-T, then wash 3 times for 5 mins with shaking, before ECL detection
ab26864 has not yet been referenced specifically in any publications.