Recombinant Anti-CD30 antibody [EPR24238-228] (ab271127)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24238-228] to CD30
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD30 antibody [EPR24238-228]
See all CD30 primary antibodies -
Description
Rabbit monoclonal [EPR24238-228] to CD30 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: KARPAS-299 and HDLM-2 cells. Flow Cyt: HDLM-2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24238-228 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/60.
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ICC/IF |
1/1000.
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Notes |
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Flow Cyt
1/60. |
ICC/IF
1/1000. |
Target
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Function
Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. -
Sequence similarities
Contains 6 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated on serine and tyrosine residues. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 943 Human
- Omim: 153243 Human
- SwissProt: P28908 Human
- Unigene: 1314 Human
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Alternative names
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
see all
Images
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized KARPAS-299 cells labelling CD30 with ab271127 at 1/1000 (0.606 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in KARPAS-299 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Negative control: HeLa (PMID: 11004694).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) preadsorbed at 1/1000 (2 ug/ml) dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HDLM-2 cells labelling CD30 with ab271127 at 1/1000 (0.606 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in HDLM-2 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) preadsorbed at 1/1000 (2 ug/ml) dilution.
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Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells (Left) and KARPAS-299 (Human anaplastic large cell lymphoma) cells (Right) labelling CD30 with ab271127 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
Negative control: Hela (PMID:11004694).
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Flow cytometric analysis of HDLM-2 (Human Hodgkin lymphoma) cells labelling CD30 with ab271127 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab271127 has not yet been referenced specifically in any publications.