RecombinantRabMAb

Recombinant Anti-CD30 antibody [EPR4102] (ab134080)

Submit a review Q&A (1)References (2)
Western blot - Anti-CD30 antibody [EPR4102] (ab134080)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)
  • Anti-CD30 antibody [EPR4102] (ab134080)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4102] to CD30
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD30 antibody [EPR4102]
    See all CD30 primary antibodies

  • Description

    Rabbit monoclonal [EPR4102] to CD30

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    corresponding to Human CD30 (C terminal).
    Database link: P28908

  • Positive control

    • IHC-P: Human Hodgkin's lymphoma and human tonsil tissue WB: KARPAS-299 whole cell lysate; HDLM-2 whole cell lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab134080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Detects a band of approximately 120 kDa (predicted molecular weight: 64 kDa).

A 90 kDa band is precursor and 120 kDa band is the mature transmembrane form as is described in PMID: 11120787.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.

  • Sequence similarities

    Contains 6 TNFR-Cys repeats.

  • Post-translational
    modifications

    Phosphorylated on serine and tyrosine residues.

  • Cellular localization

    Cytoplasm and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 30 antibody
      • CD30 antibody
      • CD30 antigen antibody
      • CD30L receptor antibody
      • Cytokine receptor CD30 antibody
      • D1S166E antibody
      • KI 1 antibody
      • KI 1 antigen antibody
      • Ki-1 antigen antibody
      • KI1 antibody
      • Lymphocyte activation antigen CD30 antibody
      • TNFRSF 8 antibody
      • Tnfrsf8 antibody
      • TNR8_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 8 antibody
      see all

    Images

    • Western blot - Anti-CD30 antibody [EPR4102] (ab134080)
      Western blot - Anti-CD30 antibody [EPR4102] (ab134080)
      All lanes : Anti-CD30 antibody [EPR4102] (ab134080) at 1/200 dilution (purified)

      Lane 1 : KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysates
      Lane 2 : HDLM-2 (Human Hodgkin lymphoma) whole cell lysates
      Lane 3 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
      Lane 4 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Developed using the ECL technique.

      Predicted band size: 64 kDa
      Observed band size: 120,90 kDa
      why is the actual band size different from the predicted?


      Exposure time: 60 seconds


      The 90 KD band is precursor and 120 KD is the mature transmembrane form as is described in PMID: 11120787.
      Raji and Daudi are negative for CD30 as is described in PMID: 29316337.
      This antibody detects high background.

      Blocking buffer and concentration:5% NFDM/TBST

      Diluting buffer and concentration:2% BSA/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD30 with purified ab134080 at 1/2000 dilution (0.82 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)

      Immunohistochemical analysis of paraffin embedded Human Hodgkin's lymphoma tissue labelling CD30 with ab134080 (unpurified) antibody at a dilution of 1/1000. Heat mediated antigen retrieval was perfomed before commencing with IHC staining protocol.

    • Anti-CD30 antibody [EPR4102] (ab134080)
      Anti-CD30 antibody [EPR4102] (ab134080)

    References (2)

    Publishing research using ab134080? Please let us know so that we can cite the reference in this datasheet.

    ab134080 has been referenced in 2 publications.

    • Yeste-Velasco M  et al. The potential of brentuximab vedotin, alone or in combination with current clinical therapies, in the treatment of testicular germ cell tumors. Am J Cancer Res 9:855-871 (2019). PubMed: 31218098
    • Rajendran S  et al. Development of a Bispecific Antibody Targeting CD30 and CD137 on Hodgkin and Reed-Sternberg Cells. Front Oncol 9:945 (2019). PubMed: 31616638

    Customer reviews and Q&As

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    Question

    Hi,   Customer is asking for concentration of ab134080 for isotype matching Please help  

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    Answer

    Thank you for your enquiry.

    I can confirm that ab134080Anti-CD30 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

    I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

    Our Rabbit IgG Isotype control ab37415 would be a good match for this product. The concentration of this control is 5mg/ml. More information may be found at:https://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html

    I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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