Recombinant
RabMAb

Recombinant Anti-CD30 antibody [EPR4102] (ab134080)

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Overview

  • Product name

    Anti-CD30 antibody [EPR4102]
    See all CD30 primary antibodies

  • Description

    Rabbit monoclonal [EPR4102] to CD30

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to residues at the C-terminus of Human CD30 (UniProt ID: P28908).

  • Positive control

    • Human Hodgkin's lymphoma tissue WB: KARPAS-299 whole cell lysate; HDLM-2 whole cell lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Detects a band of approximately 120 kDa (predicted molecular weight: 64 kDa).

A 90 kDa band is precursor and 120 kDa band is the mature transmembrane form as is described in PMID: 11120787.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.

    • Target

    • Function

      Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.

    • Sequence similarities

      Contains 6 TNFR-Cys repeats.

    • Post-translational
      modifications

      Phosphorylated on serine and tyrosine residues.

    • Cellular localization

      Cytoplasm and Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 30 antibody
        • CD30 antibody
        • CD30 antigen antibody
        • CD30L receptor antibody
        • Cytokine receptor CD30 antibody
        • D1S166E antibody
        • KI 1 antibody
        • KI 1 antigen antibody
        • Ki-1 antigen antibody
        • KI1 antibody
        • Lymphocyte activation antigen CD30 antibody
        • TNFRSF 8 antibody
        • Tnfrsf8 antibody
        • TNR8_HUMAN antibody
        • Tumor necrosis factor receptor superfamily member 8 antibody
        see all

      Images

      • Western blot - Anti-CD30 antibody [EPR4102] (ab134080)
        Western blot - Anti-CD30 antibody [EPR4102] (ab134080)
        All lanes : Anti-CD30 antibody [EPR4102] (ab134080) at 1/200 dilution

        Lane 1 : KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysates
        Lane 2 : HDLM-2 (Human Hodgkin lymphoma) whole cell lysates
        Lane 3 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
        Lane 4 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

        Developed using the ECL technique.

        Predicted band size: 64 kDa
        Observed band size: 120,90 kDa
        why is the actual band size different from the predicted?


        Exposure time: 60 seconds


        The 90 KD band is precursor and 120 KD is the mature transmembrane form as is described in PMID: 11120787.
        Raji and Daudi are negative for CD30 as is described in PMID: 29316337.
        This antibody detects high background.

        Blocking buffer and concentration:5% NFDM/TBST

        Diluting buffer and concentration:2% BSA/TBST

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (ab134080)

        Immunohistochemical analysis of paraffin embedded Human Hodgkin's lymphoma tissue labelling CD30 with ab134080 antibody at a dilution of 1/1000.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Yeste-Velasco M  et al. The potential of brentuximab vedotin, alone or in combination with current clinical therapies, in the treatment of testicular germ cell tumors. Am J Cancer Res 9:855-871 (2019). Read more (PubMed: 31218098) »
      See 1 Publication for this product

      Publishing research using ab134080? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Hi,   Customer is asking for concentration of ab134080 for isotype matching Please help  

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      Answer

      Thank you for your enquiry.

      I can confirm that ab134080Anti-CD30 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

      I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml

      Our Rabbit IgG Isotype control ab37415 would be a good match for this product. The concentration of this control is 5mg/ml. More information may be found at:https://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html

      I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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