Product nameAnti-CD30 antibody [EPR4102]
See all CD30 primary antibodies
DescriptionRabbit monoclonal [EPR4102] to CD30
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues at the C-terminus of Human CD30 (UniProt ID: P28908).
- Human Hodgkin's lymphoma tissue WB: KARPAS-299 whole cell lysate; HDLM-2 whole cell lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab134080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200. Detects a band of approximately 120 kDa (predicted molecular weight: 64 kDa).
A 90 kDa band is precursor and 120 kDa band is the mature transmembrane form as is described in PMID: 11120787.
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionReceptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
Sequence similaritiesContains 6 TNFR-Cys repeats.
modificationsPhosphorylated on serine and tyrosine residues.
Cellular localizationCytoplasm and Cell membrane.
- Information by UniProt
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
All lanes : Anti-CD30 antibody [EPR4102] (ab134080) at 1/200 dilution
Lane 1 : KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysates
Lane 2 : HDLM-2 (Human Hodgkin lymphoma) whole cell lysates
Lane 3 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lane 4 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Developed using the ECL technique.
Predicted band size: 64 kDa
Observed band size: 120,90 kDa why is the actual band size different from the predicted?
Exposure time: 60 seconds
The 90 KD band is precursor and 120 KD is the mature transmembrane form as is described in PMID: 11120787.
Raji and Daudi are negative for CD30 as is described in PMID: 29316337.
This antibody detects high background.
Blocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:2% BSA/TBST
Immunohistochemical analysis of paraffin embedded Human Hodgkin's lymphoma tissue labelling CD30 with ab134080 antibody at a dilution of 1/1000.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This product has been referenced in:
- Yeste-Velasco M et al. The potential of brentuximab vedotin, alone or in combination with current clinical therapies, in the treatment of testicular germ cell tumors. Am J Cancer Res 9:855-871 (2019). Read more (PubMed: 31218098) »