Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4102] to CD30
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-CD30 antibody [EPR4102]
See all CD30 primary antibodies
DescriptionRabbit monoclonal [EPR4102] to CD30
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
corresponding to Human CD30 (C terminal).
Database link: P28908
- IHC-P: Human Hodgkin's lymphoma and human tonsil tissue WB: KARPAS-299 whole cell lysate; HDLM-2 whole cell lysate
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab134080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200. Detects a band of approximately 120 kDa (predicted molecular weight: 64 kDa).
A 90 kDa band is precursor and 120 kDa band is the mature transmembrane form as is described in PMID: 11120787.
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionReceptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
Sequence similaritiesContains 6 TNFR-Cys repeats.
modificationsPhosphorylated on serine and tyrosine residues.
Cellular localizationCytoplasm and Cell membrane.
- Information by UniProt
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
All lanes : Anti-CD30 antibody [EPR4102] (ab134080) at 1/200 dilution (purified)
Lane 1 : KARPAS-299 (Human anaplastic large cell lymphoma) whole cell lysates
Lane 2 : HDLM-2 (Human Hodgkin lymphoma) whole cell lysates
Lane 3 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lane 4 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Developed using the ECL technique.
Predicted band size: 64 kDa
Observed band size: 120,90 kDa why is the actual band size different from the predicted?
Exposure time: 60 seconds
The 90 KD band is precursor and 120 KD is the mature transmembrane form as is described in PMID: 11120787.
Raji and Daudi are negative for CD30 as is described in PMID: 29316337.
This antibody detects high background.
Blocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:2% BSA/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD30 with purified ab134080 at 1/2000 dilution (0.82 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemical analysis of paraffin embedded Human Hodgkin's lymphoma tissue labelling CD30 with ab134080 (unpurified) antibody at a dilution of 1/1000. Heat mediated antigen retrieval was perfomed before commencing with IHC staining protocol.
ab134080 has been referenced in 2 publications.
- Yeste-Velasco M et al. The potential of brentuximab vedotin, alone or in combination with current clinical therapies, in the treatment of testicular germ cell tumors. Am J Cancer Res 9:855-871 (2019). PubMed: 31218098
- Rajendran S et al. Development of a Bispecific Antibody Targeting CD30 and CD137 on Hodgkin and Reed-Sternberg Cells. Front Oncol 9:945 (2019). PubMed: 31616638