Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23009-76] to CD30-L
- Suitable for: Flow Cyt
- Reacts with: Mouse
Related conjugates and formulations
Product nameAnti-CD30-L antibody [EPR23009-76]
See all CD30-L primary antibodies
DescriptionRabbit monoclonal [EPR23009-76] to CD30-L
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB
Species reactivityReacts with: Mouse
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
- Flow Cyt: Mouse splenocytes treated with 2.5 g/ml concanavalin A.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab267462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionCytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Sequence similaritiesBelongs to the tumor necrosis factor family.
- Information by UniProt
- CD153 antibody
- CD153 antigen antibody
- CD30 antigen ligand antibody
Flow cytometric analysis of mouse splenocytes treated with 2.5 µg/ml concanavalin A (ConA) for 24h cells labeling CD30-L with ab267462 at 1/50 dilution (Right) compared to rabbit monoclonal IgG ab172730 isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab267462 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor® 647.
Gated on viable cells.
CD30-L is expressed on activated CD4+ T cells (PMID: 10080930).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab267462 has not yet been referenced specifically in any publications.