Anti-CD300 antibody [MEM-260] (ab53317)
Key features and details
- Mouse monoclonal [MEM-260] to CD300
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD300 antibody [MEM-260]
See all CD300 primary antibodies -
Description
Mouse monoclonal [MEM-260] to CD300 -
Host species
Mouse -
Specificity
ab53317 recognizes CD300a, (Customer feedback suggests it also cross-reacts with CD300c, however we have not yet tested it for CD300c). The antibody MEM-260 reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells. -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD300. HPB human lymphoid leukemia cell line
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Positive control
- Positive material: HPB cell line. Negative material: Jurkat human cell line, Raji human cell line.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-260 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 32 kDa. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. |
Target
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Function
Acts as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation. -
Tissue specificity
Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells. -
Sequence similarities
Belongs to the CD300 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPhosphorylated on tyrosine. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 146722 Human
- Omim: 609807 Human
- SwissProt: Q8TDQ1 Human
- Unigene: 567706 Human
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Alternative names
- CD300 antigen like family member antibody
- CD300 antigen-like family member F antibody
- CD300 molecule like family member f antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab53317 has not yet been referenced specifically in any publications.