Key features and details
- Rabbit polyclonal to CD300C
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD300C antibody
See all CD300C primary antibodies
DescriptionRabbit polyclonal to CD300C
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human spleen tissue; Transfected HEK293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Tissue specificityPresent on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes and lymphocytic cell lines.
Sequence similaritiesBelongs to the CD300 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- CD300 antigen-like family member C antibody
- CD300c antibody
- CD300c antigen antibody
Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei. Recommended concentration of ab121435 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121435 at a 1/250 dilution staining CD300C in paraffin-embedded Human spleen tissue by Immunohistochemistry.
All lanes : Anti-CD300C antibody (ab121435) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T lysate
Lane 2 : CD300C transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)
Developed using the ECL technique.
ab121435 has not yet been referenced specifically in any publications.