Recombinant Anti-CD300C antibody [EPR16470] (ab195811)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16470] to CD300C
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CD300C antibody [EPR16470]
See all CD300C primary antibodies -
Description
Rabbit monoclonal [EPR16470] to CD300C -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD300C aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: Q08708 -
Positive control
- THP1, Human fetal spleen and fetal brain, Mouse heart, kidney and spleen, Rat heart and spleen, RAW 264.7, PC12 and NIH 3T3 lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16470 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab195811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa). |
Target
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Tissue specificity
Present on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes and lymphocytic cell lines. -
Sequence similarities
Belongs to the CD300 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 10871 Human
- Entrez Gene: 387565 Mouse
- Entrez Gene: 501745 Rat
- Omim: 606786 Human
- SwissProt: Q08708 Human
- SwissProt: A2A7V7 Mouse
- Unigene: 2605 Human
- Unigene: 351450 Mouse
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Alternative names
- CD300 antigen-like family member C antibody
- CD300c antibody
- CD300c antigen antibody
see all
Images
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All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat spleen lysate
Lane 6 : RAW 264.7 lysate
Lane 7 : PC12 lysate
Lane 8 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 25 kDaThe observed MW is consistent with what has been described in the literature (PMID:20656921)
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All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 25 kDaThe observed MW is consistent with what has been described in the literature (PMID:20656921)
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Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution + THP1 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 25 kDaThe observed MW is consistent with what has been described in the literature (PMID:20656921)
Datasheets and documents
Certificate of Compliance
References (0)
ab195811 has not yet been referenced specifically in any publications.