Recombinant
RabMAb

Recombinant Anti-CD300C antibody [EPR16470] (ab195811)

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Overview

  • Product name

    Anti-CD300C antibody [EPR16470]
    See all CD300C primary antibodies

  • Description

    Rabbit monoclonal [EPR16470] to CD300C

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD300C aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q08708

  • Positive control

    • THP1, Human fetal spleen and fetal brain, Mouse heart, kidney and spleen, Rat heart and spleen, RAW 264.7, PC12 and NIH 3T3 lysates; Jurkat cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16470

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa).
ICC/IF 1/100.

Target

  • Tissue specificity

    Present on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes and lymphocytic cell lines.

  • Sequence similarities

    Belongs to the CD300 family.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD300 antigen-like family member C antibody
      • CD300c antibody
      • CD300c antigen antibody
      • CD300c molecule antibody
      • CLM-6 antibody
      • CLM6_HUMAN antibody
      • CMRF-35 antibody
      • CMRF-35A antibody
      • CMRF35 antibody
      • CMRF35 antigen antibody
      • CMRF35 leukocyte immunoglobulin-like receptor antibody
      • CMRF35-A1 antibody
      • CMRF35-like molecule 6 antibody
      • CMRF35A antibody
      • CMRF35A leukocyte immunoglobulin-like receptor antibody
      • CMRF35A1 antibody
      • IgSF16 antibody
      • Immunoglobulin superfamily member 16 antibody
      • LIR antibody
      see all

    Images

    • Western blot - Anti-CD300C antibody [EPR16470] (ab195811)
      Western blot - Anti-CD300C antibody [EPR16470] (ab195811)
      All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution

      Lane 1 : Mouse heart lysate
      Lane 2 : Mouse kidney lysate
      Lane 3 : Mouse spleen lysate
      Lane 4 : Rat heart lysate
      Lane 5 : Rat spleen lysate
      Lane 6 : RAW 264.7 lysate
      Lane 7 : PC12 lysate
      Lane 8 : NIH 3T3 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 25 kDa



      The observed MW is consistent with what has been described in the literature (PMID:20656921)

    • Western blot - Anti-CD300C antibody [EPR16470] (ab195811)
      Western blot - Anti-CD300C antibody [EPR16470] (ab195811)
      All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution

      Lane 1 : Human fetal spleen lysate
      Lane 2 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 25 kDa



      The observed MW is consistent with what has been described in the literature (PMID:20656921)

    • Western blot - Anti-CD300C antibody [EPR16470] (ab195811)
      Western blot - Anti-CD300C antibody [EPR16470] (ab195811)
      Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution + THP1 cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 25 kDa



      The observed MW is consistent with what has been described in the literature (PMID:20656921)

    References

    ab195811 has not yet been referenced specifically in any publications.

    Publishing research using ab195811? Please let us know so that we can cite the reference in this datasheet.

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