Recombinant Anti-CD300C antibody [EPR16470] - BSA and Azide free (ab251213)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16470] to CD300C - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD300C antibody [EPR16470] - BSA and Azide free
See all CD300C primary antibodies -
Description
Rabbit monoclonal [EPR16470] to CD300C - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251213 is the carrier-free version of ab195811.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16470 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa). |
Target
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Tissue specificity
Present on the surface of monocytes, neutrophils, a proportion of peripheral blood T- and B-lymphocytes and lymphocytic cell lines. -
Sequence similarities
Belongs to the CD300 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 10871 Human
- Entrez Gene: 387565 Mouse
- Entrez Gene: 501745 Rat
- Omim: 606786 Human
- SwissProt: Q08708 Human
- SwissProt: A2A7V7 Mouse
- Unigene: 2605 Human
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Alternative names
- CD300 antigen-like family member C antibody
- CD300c antibody
- CD300c antigen antibody
see all
Images
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All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : Rat spleen tissue lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 lysate
Lane 8 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 25 kDaThis data was developed using ab195811, the same antibody clone in a different buffer formulation.
The observed MW is consistent with what has been described in the literature (PMID:20656921).
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All lanes : Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution
Lane 1 : Human fetal spleen lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 25 kDaThis data was developed using ab195811, the same antibody clone in a different buffer formulation.
The observed MW is consistent with what has been described in the literature (PMID:20656921).
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Anti-CD300C antibody [EPR16470] (ab195811) at 1/1000 dilution + THP1 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 25 kDaThis data was developed using ab195811, the same antibody clone in a different buffer formulation.
The observed MW is consistent with what has been described in the literature (PMID: 20656921).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251213 has not yet been referenced specifically in any publications.