Anti-CD31 antibody (ab28364)
Key features and details
- Rabbit polyclonal to CD31
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD31 antibody
See all CD31 primary antibodies -
Description
Rabbit polyclonal to CD31 -
Host species
Rabbit -
Specificity
Customer feedback indicates that there might be some batch-to-batch variation with respect to mouse cross-reactivity. We recommend ab124432 as an alternative antibody for mouse samples. Please contact Abcam if you have any queries. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Pig
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Immunogen
Synthetic peptide within Mouse CD31 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q08481 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab28364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue |
Target
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Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
Tissue specificity
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
Sequence similarities
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
Post-translational
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
Cellular localization
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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Database links
- Entrez Gene: 5175 Human
- Entrez Gene: 18613 Mouse
- Omim: 173445 Human
- SwissProt: P16284 Human
- SwissProt: Q08481 Mouse
- Unigene: 376675 Human
- Unigene: 514412 Human
- Unigene: 343951 Mouse
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Alternative names
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)This image is courtesy of an Abreview submitted by Mr. Victor Bernal.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD31 with ab28364 at 1/50 dilution (30 minutes at 23°C). 10% Neutral buffered formalin used as fixative. Heat mediated antigen retrieval was performed at 95°C for 20 minutes. Blocking performed using 2.5% serum for 20 minutes at 23°C. HRP-conjugated Goat Anti-rabbit was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)This image is courtesy of an anonymous Abreview.Immunohistochemistry (PFA-fixed paraffin-embedded sections) analysis of human palatine tonsil tissue labelling CD31 with ab28364 at 1/50 dilution (15 hours at 4°C). Heat mediated antigen retrieval was performed using Tris/EDTA buffer. Blocking performed with 5% normal goat serum for 1 hour at 21°C. HRP-conjugated Goat Anti-Rabbit was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
Protocols
References (1173)
ab28364 has been referenced in 1173 publications.
- Stock AA et al. Conformal Coating of Stem Cell-Derived Islets for ß Cell Replacement in Type 1 Diabetes. Stem Cell Reports 14:91-104 (2020). PubMed: 31839542
- Barbeito-Andrés J et al. Congenital Zika syndrome is associated with maternal protein malnutrition. Sci Adv 6:eaaw6284 (2020). PubMed: 31950075
- Ayanlaja AA et al. Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression. Cell Commun Signal 18:24 (2020). PubMed: 32050972
- Rutkai I et al. Chronic imaging of mitochondria in the murine cerebral vasculature using in vivo two-photon microscopy. Am J Physiol Heart Circ Physiol 318:H1379-H1386 (2020). PubMed: 32330090
- Luo S et al. Targeting self-assembly peptide for inhibiting breast tumor progression and metastasis. Biomaterials 249:120055 (2020). PubMed: 32315863
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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