Overview

  • Product name
  • Description
    Rabbit polyclonal to CD31
  • Host species
    Rabbit
  • Specificity
    Customer feedback indicates that there might be some batch-to-batch variation with respect to mouse cross-reactivity. We recommend ab124432 as an alternative antibody for mouse samples. Please contact Abcam if you have any queries.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Synthetic peptide within Mouse CD31 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q08481

  • Positive control
    • IHC-P: Mouse skin, xenograft tissue; Human tonsil tissue. WB: Jurkat whole cell lysate (ab7899), Human vascular endothelial cell lysate. IHC-Fr: Human fetal heart tissue.
  • General notes

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

Applications

Our Abpromise guarantee covers the use of ab28364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/20. PubMed: 17316380
IHC-FoFr Use at an assay dependent concentration.
WB 1/500.

Target

  • Function
    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • Tissue specificity
    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • Sequence similarities
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain
    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • Post-translational
    modifications
    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • Cellular localization
    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

Images

  • Representative images of cross sections with CD31 staining in red, nucleus in blue. White dotted lines indicate the graft wall. Scale bar = 100 μm.

    Explanted grafts from C57/BL6 mice were fixed overnight in paraformaldehyde (2%) at room temperature. Samples were dehydrated through an ethanol gradient and embedded in paraffin and sectioned at 5 μm transversely from proximal anastomosis to distal anastomosis. For immunohistochemistry staining, sections were deparaffinised and stained with antibody against CD31 (Abcam, ab28364, 1:200) for endothelial cells.

     

  • GFP positive cells (green) were colocalized with CD31 (red). Nuclear staining with DAPI is blue.

    For immunofluorescence staining, the skin samples were fixed in 4% paraformaldehyde and embedded in paraffin. Skin sections were stained with an anti-GFP antibody. The skin sections were also treated with primary Abs against CD31 (Abcam, ab28364, diluted 1:300). Secondary antibodies conjugated to rhodamine-isothiocyanate were used for fluorescence detection on a confocal laser scanning fluorescence microscope (Zeiss, German).

    Opn-/-; OPN (Osteopontin) knock-out mice.

  • Anti-CD31 antibody (ab28364) at 1/500 dilution + Human vascular endothelial cells at 25 µg

    Secondary
    anti-rabbit HRP at 1/5000 dilution

    See Abreview

  • ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • ab28364 staining CD31 in mouse xenograft tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices and blocked with 1% BSA for 60 minutes at 21°C. Antigen retrieval was by heat mediation in a Tris/EDTA pH9 buffer. The sample was incubated with primary antibody (20 ug/mL) at 4°C for 16 hours. A HRP-conjugated goat anti-rabbit polyclonal (2 ug/mL) was used as the secondary antibody.

    See Abreview

  • ab28364 at a 1/300 dilution, staining human CD31 in fetal heart tissue by Immunohistochemistry, frozen tissue.

    See Abreview

  • Anti-CD31 antibody (ab28364) at 1/50 dilution staining mouse tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was formaldehyde fixed and blocked with serum. The tissue was incubated with the primary antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary. The endothelium of vessels of any tissues are positive.

    See Abreview

References

This product has been referenced in:
  • Xu L  et al. ATM deficiency promotes progression of CRPC by enhancing Warburg effect. Endocr Relat Cancer 26:59-71 (2019). Read more (PubMed: 30400006) »
  • Gallina D & Lincoln J Dynamic Expression Profiles of Sox9 in Embryonic, Post Natal, and Adult Heart Valve Cell Populations. Anat Rec (Hoboken) 302:108-116 (2019). Read more (PubMed: 30412364) »
See all 817 Publications for this product

Customer reviews and Q&As

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1-10 of 117 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (liver, endothelial)
Permeabilization
No
Specification
liver, endothelial
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Miss. Kara Shumansky

Verified customer

Submitted Mar 11 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: cirtric acid
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Miss. Jin Young Park

Verified customer

Submitted Nov 12 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (skin)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Miss. Jin Young Park

Verified customer

Submitted Nov 12 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (artery)
Permeabilization
Yes - 0.1% TX-100
Specification
artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% formalin

Abcam user community

Verified customer

Submitted Oct 29 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Endothelial cells)
Gel Running Conditions
Reduced Denaturing (8% SDS page gel)
Loading amount
10 µg
Treatment
10ng/ml VEGF for 24hr
Specification
Human Endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 24 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph 9
Permeabilization
No
Specification
brain tissue
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Formaldehyde

Herr Dr. Markus Kipp

Verified customer

Submitted Sep 07 2018

Application
IHC - Wholemount
Sample
Mouse Tissue (intestine organoids)
Specification
intestine organoids

Abcam user community

Verified customer

Submitted Jul 11 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 (EDTA pH8.5)
Permeabilization
No
Specification
Placenta
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 06 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted May 07 2018

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (P21 Mouse Brain Sections)
Specification
P21 Mouse Brain Sections

Dr. Bhavin Shah

Verified customer

Submitted Apr 17 2018

1-10 of 117 Abreviews

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