Recombinant
RabMAb

Recombinant Anti-CD31 antibody [EP3095] (ab134168)

Overview

  • Product name
    Anti-CD31 antibody [EP3095]
    See all CD31 primary antibodies
  • Description
    Rabbit monoclonal [EP3095] to CD31
  • Host species
    Rabbit
  • Specificity
    This antibody gave positive results in mouse and rat tissue staining via IHC-P, but we have had difficulties obtaining consistent positive results in WB with mouse and rat samples. Thus, at this time we unfortunately cannot guarantee this antibody to recognize mouse and rat CD31, and optimization may be necessary when working with these species.
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD31 aa 700-800. The exact sequence is proprietary.

  • Positive control
    • Human tonsil and uterus tissues; THP1 and Jurkat whole cell lysate (ab7899).
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab134168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 82 kDa.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • Tissue specificity
      Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • Sequence similarities
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain
      The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • Post-translational
      modifications
      Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • Cellular localization
      Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adhesion molecule antibody
      • CD31 antibody
      • CD31 antigen antibody
      • CD31 EndoCAM antibody
      • EndoCAM antibody
      • FLJ34100 antibody
      • FLJ58394 antibody
      • GPIIA antibody
      • GPIIA' antibody
      • PECA1 antibody
      • PECA1_HUMAN antibody
      • Pecam 1 antibody
      • PECAM 1 CD31 EndoCAM antibody
      • PECAM antibody
      • PECAM-1 antibody
      • Pecam1 antibody
      • Platelet and endothelial cell adhesion molecule 1 antibody
      • Platelet endothelial cell adhesion molecule antibody
      • Platelet/endothelial cell adhesion molecule 1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human uterus tissue, staining CD31 using ab134168 at a 1/250 dilution. Note membrane staining of endothelial cells.
    • Overlay histogram showing Jurkat cells stained with ab134168 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134168, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-CD31 antibody [EP3095] (ab134168) at 1/1000 dilution

      Lane 1 : THP1 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 82 kDa

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, staining CD31 using ab134168 at a 1/250 dilution. Note membrane staining of endothelial cells.
    • Immunohistochemical analysis of paraffin embedded normal Human spleen vessels tissue using ab134168 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Breast carcinoma vessels tissue using ab134168 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Clear cell carcinoma of kidney tissue using ab134168 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human lung tissue using ab134168 showing +ve staining.

    References

    This product has been referenced in:
    • Tan ZB  et al. Rheum palmatum extract exerts anti-hepatocellular carcinoma effects by inhibiting signal transducer and activator of transcription 3 signaling. J Ethnopharmacol 232:62-72 (2019). Read more (PubMed: 30553869) »
    • Zhang H  et al. High expression of leucine-rich repeat-containing 8A is indicative of a worse outcome of colon cancer patients by enhancing cancer cell growth and metastasis. Oncol Rep 40:1275-1286 (2018). Read more (PubMed: 30015914) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Tumour)
    Antigen retrieval step
    Heat mediated
    Specification
    Tumour
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 20 2018

    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (Hemangiosarcoma cell line)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    50 µg
    Specification
    Hemangiosarcoma cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Holly Pondenis

    Verified customer

    Submitted May 05 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ph6 citrate
    Sample
    Human Tissue sections (Human Lung Tumour)
    Specification
    Human Lung Tumour
    Permeabilization
    No
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 05 2015

    Western blot

    Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (lung)
    Specification
    lung
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted May 23 2013

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