RecombinantRabMAb

Recombinant Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

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Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
  • Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
  • Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17260-254] to CD31 - Low endotoxin, Azide free
  • Suitable for: WB, Sandwich ELISA, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free
    See all CD31 primary antibodies

  • Description

    Rabbit monoclonal [EPR17260-254] to CD31 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Sandwich ELISA, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment aa 400-600. The exact sequence is proprietary.
    Database link: Q08481

  • Positive control

    • WB: Mouse CD31 active protein; mouse and rat platelet and lung lysates; bEnd.3 whole cell lysate. IP: bEnd.3 whole cell lysate.

  • General notes

    ab224260 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab224260 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.

The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.

Ms Isoform 1-4: 69.8-81.3 kDa (predicted)

Observed band size is around 130 kDa

Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate.

Negative Control: NIH/3T3 whole cell lysate (ab7179).
Sandwich ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

  • Tissue specificity

    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).

  • Sequence similarities

    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

  • Domain

    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.

  • Post-translational
    modifications

    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.

  • Cellular localization

    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt

  • Database links

    • Alternative names

      • Adhesion molecule antibody
      • CD31 antibody
      • CD31 antigen antibody
      • CD31 EndoCAM antibody
      • EndoCAM antibody
      • FLJ34100 antibody
      • FLJ58394 antibody
      • GPIIA antibody
      • GPIIA' antibody
      • PECA1 antibody
      • PECA1_HUMAN antibody
      • Pecam 1 antibody
      • PECAM 1 CD31 EndoCAM antibody
      • PECAM antibody
      • PECAM-1 antibody
      • Pecam1 antibody
      • Platelet and endothelial cell adhesion molecule 1 antibody
      • Platelet endothelial cell adhesion molecule antibody
      • Platelet/endothelial cell adhesion molecule 1 antibody
      see all

    Images

    • Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
      Immunoprecipitation - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

      CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with ab213175 at 1/40 dilution.

      Western blot was performed from the immunoprecipitate using ab213175 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: bEnd.3 whole cell lysate 10µg (Input). Lane 2: ab213175 IP in bEnd.3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213175 in bEnd.3 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 10 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213175).

    • Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
      Sandwich ELISA - Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

      This ELISA data was generated using the same anti-CD31 antibody clone [EPR17260-254] in a different buffer formulation (cat# ab213175).

      Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.

      For a matched antibody pair set, see Mouse CD31 Matched Antibody Pair Kit (ab212065).

      For a complete ELISA kit, see Mouse CD31 ELISA kit (ab204527).

    • Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)
      Anti-CD31 antibody [EPR17260-254] - Low endotoxin, Azide free (ab224260)

    References (0)

    Publishing research using ab224260? Please let us know so that we can cite the reference in this datasheet.

    ab224260 has not yet been referenced specifically in any publications.

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