Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17260-265] to CD31
- Suitable for: WB, IP
- Reacts with: Mouse
Product nameAnti-CD31 antibody [EPR17260-265]
See all CD31 primary antibodies
DescriptionRabbit monoclonal [EPR17260-265] to CD31
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse CD31 aa 400-600. The exact sequence is proprietary.
Database link: Q08481
- WB: His-tagged mouse CD31 recombinant protein (aa18-590); bEnd.3 cell lysate; mouse platelet and lung lysate. IP: bEnd.3 whole cell lysate.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 110-130 kDa (predicted molecular weight: 81 kDa).|
FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
- Information by UniProt
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
Lane 1 : Anti-CD31 antibody [EPR17260-265] (ab182982) at 1/20000 dilution
Lane 2 : Anti-CD31 antibody [EPR17260-265] (ab182982) at 1/100000 dilution
All lanes : His-tagged mouse CD31 recombinant protein (aa18-590) 10 ng
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Lanes 1-2 : Anti-CD31 antibody [EPR17260-265] (ab182982) at 1/1000 dilution
Lane 3 : Anti-CD31 antibody [EPR17260-265] (ab182982) at 1/2000 dilution
Lane 1 : Mouse platelet lysate
Lane 2 : Mouse lung lysate
Lane 3 : bEnd.3 (mouse brain endothelioma), whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 110-130 kDa why is the actual band size different from the predicted?
Blocking: 5% NFDM /TBST.
Lane 1: 3 minutes
Lanes 2-3: 1 second
The expression MW observed is consistent with what has been described in the literature (PMID: 18388311; 12433657).
CD31 was immunoprecipitated from 1 mg bEnd.3 (mouse brain endothelioma cell line) whole cell lysate with ab182982 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab182982 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: bEnd.3 whole cell lysate 10 μg (Input).
Lane 2: ab182982 IP in bEnd.3 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab182982 in bEnd.3 whole cell lysate (-)
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab182982 has been referenced in 1 publication.
- Aleithe S et al. Transcriptional Response and Morphological Features of the Neurovascular Unit and Associated Extracellular Matrix After Experimental Stroke in Mice. Mol Neurobiol 56:7631-7650 (2019). PubMed: 31089963