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RecombinantRabMAb

Recombinant Anti-CD31 antibody [EPR3094] (ab76533)

  • Datasheet
  • SDS
Reviews (12)Q&A (2)References (36)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)
  • Immunocytochemistry - Anti-CD31 antibody [EPR3094] (ab76533)
  • Western blot - Anti-CD31 antibody [EPR3094] (ab76533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)
  • Western blot - Anti-CD31 antibody [EPR3094] (ab76533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)
  • OI-RD Scanning - Anti-CD31 antibody [EPR3094] (ab76533)
  • Anti-CD31 antibody [EPR3094] (ab76533)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3094] to CD31
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD31 antibody [EPR3094]
    See all CD31 primary antibodies
  • Description

    Rabbit monoclonal [EPR3094] to CD31
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 and Jurkat cell lysates IHC-P: Human kidney tissue and human muscle tissue ICC: THP-1 cell lysate.
  • General notes

    This antibody shows no cross-reactivity with rat and mouse samples in WB. However it can give some non specific staining on mouse smooth muscle tissues. Plesae contact our Scientific support for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Dissociation constant (KD)

    KD = 1.79 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3094
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cancer
    • Tumor immunology
    • CD markers
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Thrombocytic Lineage
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Biotin Anti-CD31 antibody [EPR3094] (ab199734)
    • Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)
    • Alexa Fluor® 647 Anti-CD31 antibody [EPR3094] (ab218582)
    • Alexa Fluor® 488 Anti-CD31 antibody [EPR3094] (ab275989)
    • Alexa Fluor® 594 Anti-CD31 antibody [EPR3094] (ab277231)
    • Alexa Fluor® 555 Anti-CD31 antibody [EPR3094] (ab279331)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human CD31 protein (ab155607)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC (2)
1/500.

It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen. Use 0.3M glycine to quench autofluorescence caused by aldehydes.

Positive Control: HUVEC cells

WB (1)
1/5000 - 1/20000. Predicted molecular weight: 83 kDa.

Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.

The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.

Hu Isoform 1-6: 79-83 kDa (predicted)

Observed band size is around 130 kDa

Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate.

Negative Control: NIH/3T3 whole cell lysate (ab7179).

IHC-P (7)
1/100 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

We suggest to program the pressure cooker to run for 30 seconds at 125° C, followed by 10 seconds at 90° C. Then let the slide cool down to room temperature (10 - 20 minutes).

The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples.

Positive Control: Hu tonsil tissue

Notes
ICC
1/500.

It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen. Use 0.3M glycine to quench autofluorescence caused by aldehydes.

Positive Control: HUVEC cells

WB
1/5000 - 1/20000. Predicted molecular weight: 83 kDa.

Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary.

The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31.

Hu Isoform 1-6: 79-83 kDa (predicted)

Observed band size is around 130 kDa

Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate.

Negative Control: NIH/3T3 whole cell lysate (ab7179).

IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

We suggest to program the pressure cooker to run for 30 seconds at 125° C, followed by 10 seconds at 90° C. Then let the slide cool down to room temperature (10 - 20 minutes).

The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples.

Positive Control: Hu tonsil tissue

  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • Tissue specificity

      Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • Sequence similarities

      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • Domain

      The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • Post-translational
      modifications

      Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • Cellular localization

      Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Target information above from: UniProt accession P16284 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5175 Human
      • Omim: 173445 Human
      • SwissProt: P16284 Human
      • Unigene: 376675 Human
      • Unigene: 514412 Human
      • Alternative names

        • Adhesion molecule antibody
        • CD31 antibody
        • CD31 antigen antibody
        • CD31 EndoCAM antibody
        • EndoCAM antibody
        • FLJ34100 antibody
        • FLJ58394 antibody
        • GPIIA antibody
        • GPIIA' antibody
        • PECA1 antibody
        • PECA1_HUMAN antibody
        • Pecam 1 antibody
        • PECAM 1 CD31 EndoCAM antibody
        • PECAM antibody
        • PECAM-1 antibody
        • Pecam1 antibody
        • Platelet and endothelial cell adhesion molecule 1 antibody
        • Platelet endothelial cell adhesion molecule antibody
        • Platelet/endothelial cell adhesion molecule 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)

        ab76533 staining CD31 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

         

      • Immunocytochemistry - Anti-CD31 antibody [EPR3094] (ab76533)
        Immunocytochemistry - Anti-CD31 antibody [EPR3094] (ab76533)
        Immunocytochemistry analysis of THP-1 (Human monocytic leukemia monocyte) cell line labeling CD31 with ab76533 at 1/250 (4.9 μg/mL). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used as a counterstain at 1/200 dilution. DAPI (blue) was used as nuclear counterstain.

        Confocal image showing membranous staining in THP-1 cells. 
      • Western blot - Anti-CD31 antibody [EPR3094] (ab76533)
        Western blot - Anti-CD31 antibody [EPR3094] (ab76533)
        Anti-CD31 antibody [EPR3094] (ab76533) at 1/10000 dilution + THP-1 (Human monocytic leukemia cell line) whole cell lysate at 20 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 83 kDa
        Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.



        Blocking and diluting buffer: 5% NFDM /TBST 

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)Image from Hofmann NA et al., PLoS One. 2012;7(9):e44468. doi: 10.1371/journal.pone.0044468. Epub 2012 Sep 7. Fig 3.; doi:10.1371/journal.pone.0044468; September 7, 2012, PLoS ONE 7(9): e44468.
        Immunohistochemical analysis of endothelial colony forming progenitor cell plugs, staining CD31 (green) with ab76533.

        Following antigen retrieval and blocking, sections were incubated with primary antibody (1/1000) overnight at 4°C. A Cy5®-conjugated anti-rabbit IgG (2 µg/ml) was used as the secondary antibody.
      • Western blot - Anti-CD31 antibody [EPR3094] (ab76533)
        Western blot - Anti-CD31 antibody [EPR3094] (ab76533)
        All lanes : Anti-CD31 antibody [EPR3094] (ab76533) at 1/20000 dilution

        Lane 1 : THP-1 cell lysate
        Lane 2 : Jurkat cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 83 kDa
        Observed band size: 125 kDa why is the actual band size different from the predicted?

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)

        Immunohistochemical analysis of paraffin embedded human kidney tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)

        Immunohistochemical analysis of paraffin embedded human muscle tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] (ab76533)Image courtesy of an anonymous Abreview.
        ab76533 staining CD31 in human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 3% serum for 30 minutes at 20°C followed by incubation with the primary antibody at a 1/200 dilution for 12 hours at20°C. A Cy3®-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution.

        See Abreview

      • OI-RD Scanning - Anti-CD31 antibody [EPR3094] (ab76533)
        OI-RD Scanning - Anti-CD31 antibody [EPR3094] (ab76533)

        Equilibrium disassociation constant (KD) measurement to determine antibody affinity to the target antigen.

        Click here to learn more about KD

      • Anti-CD31 antibody [EPR3094] (ab76533)
        Anti-CD31 antibody [EPR3094] (ab76533)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (36)

      Publishing research using ab76533? Please let us know so that we can cite the reference in this datasheet.

      ab76533 has been referenced in 36 publications.

      • Wang L  et al. Homozygous MESP1 knock-in reporter hESCs facilitated cardiovascular cell differentiation and myocardial infarction repair. Theranostics 10:6898-6914 (2020). PubMed: 32550911
      • Jiang Z  et al. Inactivation of the Wnt/ß-catenin signaling pathway underlies inhibitory role of microRNA-129-5p in epithelial-mesenchymal transition and angiogenesis of prostate cancer by targeting ZIC2. Cancer Cell Int 19:271 (2019). PubMed: 31649488
      • Lefley D  et al. Development of clinically relevant in vivo metastasis models using human bone discs and breast cancer patient-derived xenografts. Breast Cancer Res 21:130 (2019). PubMed: 31783893
      • Sato S  et al. EPHB2 carried on small extracellular vesicles induces tumor angiogenesis via activation of ephrin reverse signaling. JCI Insight 4:N/A (2019). PubMed: 31661464
      • Sun C  et al. Meis2 represses the osteoblastic transdifferentiation of aortic valve interstitial cells through the Notch1/Twist1 pathway. Biochem Biophys Res Commun 509:455-461 (2019). PubMed: 30594396
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-10 of 12 Abreviews

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [EPR3094]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Frontal Cortex (Brain))
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA
      Permeabilization
      Yes - .1% TritonX-100
      Specification
      Frontal Cortex (Brain)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Mr. Daniel Kirsch

      Verified customer

      Submitted Nov 30 2020

      Immunocytochemistry abreview for Anti-CD31 antibody [EPR3094]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cell (Kidney H9 Organoids)
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney H9 Organoids
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 24 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [EPR3094]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Cirrhosis Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Cirrhosis Liver
      Blocking step
      sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 31 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [EPR3094]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (MDA-MB-231 xenograft model)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citric acid
      Permeabilization
      No
      Specification
      MDA-MB-231 xenograft model
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 01 2017

      Immunocytochemistry abreview for Anti-CD31 antibody [EPR3094]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cell (HeLa)
      Permeabilization
      Yes - 0.5% Triton X-100 in PBS
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Jan 16 2017

      Immunohistochemistry (Frozen sections) abreview for Anti-CD31 antibody [EPR3094]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Muscle)
      Permeabilization
      Yes - Triton and Tween
      Specification
      Muscle
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 27 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [EPR3094]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citric acid pH6
      Permeabilization
      No
      Specification
      Kidney
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Mr. Carl Hobbs

      Verified customer

      Submitted Sep 06 2016

      Immunohistochemistry (Frozen sections) abreview for Anti-CD31 antibody [EPR3094]

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Skin)
      Permeabilization
      No
      Specification
      Skin
      Blocking step
      (agent) for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
      Fixative
      Acetone
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 21 2015

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [EPR3094]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
      Sample
      Pig Tissue sections (skin)
      Specification
      skin
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 18 2015

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 [EPR3094] antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6
      Sample
      Human Tissue sections (muscle)
      Specification
      muscle
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 23 2013

      1-10 of 12 Abreviews

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