Recombinant
RabMAb

Recombinant Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3094] to CD31 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD31 antibody [EPR3094] - BSA and Azide free
    See all CD31 primary antibodies

  • Description

    Rabbit monoclonal [EPR3094] to CD31 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to residues in the extracellular domain of human CD31

  • Positive control

    • WB: THP-1 and Jurkat cell lysates IHC-P: Human kidney tissue and human muscle tissue ICC/IF: Jurkat cells

  • General notes

    ab207090 is the carrier-free version of ab76533 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab207090 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody shows no cross-reactivity with rat and mouse samples in WB. However it can give some non specific staining on mouse smooth muscle tissues. Plesae contact our Scientific support for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

Our Abpromise guarantee covers the use of ab207090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

    • Tissue specificity

      Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).

    • Sequence similarities

      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

    • Domain

      The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.

    • Post-translational
      modifications

      Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.

    • Cellular localization

      Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Information by UniProt

    • Database links

      • Alternative names

        • Adhesion molecule antibody
        • CD31 antibody
        • CD31 antigen antibody
        • CD31 EndoCAM antibody
        • EndoCAM antibody
        • FLJ34100 antibody
        • FLJ58394 antibody
        • GPIIA antibody
        • GPIIA' antibody
        • PECA1 antibody
        • PECA1_HUMAN antibody
        • Pecam 1 antibody
        • PECAM 1 CD31 EndoCAM antibody
        • PECAM antibody
        • PECAM-1 antibody
        • Pecam1 antibody
        • Platelet and endothelial cell adhesion molecule 1 antibody
        • Platelet endothelial cell adhesion molecule antibody
        • Platelet/endothelial cell adhesion molecule 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)

        ab76533 staining CD31 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76533).

      • Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)
        Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)
        Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling CD31 with ab76533 at 1/500 (4.9 μg/mL). ab150077, AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/mL) was used as the secondary antibody. Cells were fixed with 100% Methanol. DAPI (blue) was used as nuclear counterstain.

        Confocal image showing positive staining on Jurkat cells.
         
        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76533).

         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)

        Immunohistochemical analysis of paraffin embedded human kidney tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76533).

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)

        Immunohistochemical analysis of paraffin embedded human muscle tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76533).

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)This image is courtesy of an anonymous Abreview.
        ab76533 staining CD31 in human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 3% serum for 30 minutes at 20°C followed by incubation with the primary antibody at a 1/200 dilution for 12 hours at20°C. A Cy3®-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76533).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)Image from Hofmann NA et al. PLoS One. 2012;7(9):e44468. doi: 10.1371/journal.pone.0044468. Epub 2012 Sep 7. Fig 3.; doi:10.1371/journal.pone.0044468; September 7 2012 PLoS ONE 7(9): e44468.
        Immunohistochemical analysis of endothelial colony forming progenitor cell plugs, staining CD31 (green) with ab76533.

        Following antigen retrieval and blocking, sections were incubated with primary antibody (1/1000) overnight at 4°C. A Cy5®-conjugated anti-rabbit IgG (2 µg/ml) was used as the secondary antibody.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76533).

      • Other - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)
        Other - Anti-CD31 antibody [EPR3094] - BSA and Azide free (ab207090)

        Equilibrium disassociation constant (KD) measurement to determine antibody affinity to the target antigen.

        Click here to learn more about KD

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76533).

      References

      Publishing research using ab207090? Please let us know so that we can cite the reference in this datasheet.

      ab207090 has been referenced in 6 publications.

      • Xue Y  et al. Tumor-infiltrating M2 macrophages driven by specific genomic alterations are associated with prognosis in bladder cancer. Oncol Rep 42:581-594 (2019). PubMed: 31233191
      • Ren L  et al. Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration. Biomed Res Int 2014:301279 (2014). IHC-P, ICC/IF ; Mouse, Human . PubMed: 25110670
      • He KF  et al. CD163+ tumor-associated macrophages correlated with poor prognosis and cancer stem cells in oral squamous cell carcinoma. Biomed Res Int 2014:838632 (2014). IHC-P ; Human . PubMed: 24883329
      • Shu Q  et al. Vasostatin inhibits VEGF-induced endothelial cell proliferation, tube formation and induces cell apoptosis under oxygen deprivation. Int J Mol Sci 15:6019-30 (2014). ICC/IF ; Human . PubMed: 24722573
      • Hofmann NA  et al. Oxygen sensing mesenchymal progenitors promote neo-vasculogenesis in a humanized mouse model in vivo. PLoS One 7:e44468 (2012). IHC-P ; Human . PubMed: 22970226
      • Balcells M  et al. Smooth muscle cells orchestrate the endothelial cell response to flow and injury. Circulation 121:2192-9 (2010). PubMed: 20458015

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