Key features and details
- Mouse monoclonal [JC/70A] to CD31 - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD31 antibody [JC/70A] - BSA and Azide free
See all CD31 primary antibodies
DescriptionMouse monoclonal [JC/70A] to CD31 - BSA and Azide free
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cyt, IHC-Frmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cynomolgus monkeyDoes not react with: Rat, Pig
Tissue, cells or virus corresponding to Human CD31. Membrane preparation of a spleen from a patient with hairy cell leukemia.
Database link: P16284
- Human colon carcinoma and Human Angiosarcoma tissues; Jurkat cells. Tonsil
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A/G purified
Light chain typekappa
- Anti-CD31 antibody [JC/70A] (PE) (ab215660)
- Anti-CD31 antibody [JC/70A] (HRP) (ab215774)
- Anti-CD31 antibody [JC/70A] (Alexa Fluor® 488) (ab215911)
- Anti-CD31 antibody [JC/70A] (Alexa Fluor® 647) (ab215912)
- Anti-CD31 antibody [JC/70A] - BSA and Azide free (ab264090)
- Anti-CD31 antibody [JC/70A] (ab9498)
Our Abpromise guarantee covers the use of ab212712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 82 kDa.|
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|Flow Cyt||Use 0.5-1µg for 106 cells.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
- Information by UniProt
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
ab212712 has not yet been referenced specifically in any publications.