Anti-CD31 antibody [MEC 7.46] (ab7388)
![Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (ab7388)](https://www.abcam.com/ps/products/7/ab7388/Images/ab7388-275753-anti-cd31-antibody-mec-746-flow-cytometry.jpg "Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (ab7388)")
Key features and details
- Rat monoclonal [MEC 7.46] to CD31
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-CD31 antibody [MEC 7.46]
See all CD31 primary antibodies -
Description
Rat monoclonal [MEC 7.46] to CD31 -
Host species
Rat -
Specificity
The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cell presents on all mouse blood vessels both in normal and inflamed or tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Tissue, cells or virus corresponding to CD31.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered antibody solution. -
Clonality
Monoclonal -
Clone number
MEC 7.46 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
No fixation or permeabilization needed. Epitope is sensitive to trypsin! |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. No fixation or permeabilization needed. Epitope is sensitive to trypsin! |
Application notes
Is unsuitable for IHC-P.
Target
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Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
Tissue specificity
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
Sequence similarities
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
Post-translational
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
Cellular localization
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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Database links
- Entrez Gene: 18613 Mouse
- SwissProt: Q08481 Mouse
- Unigene: 343951 Mouse
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Alternative names
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
Images
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Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (ab7388)
Flow Cytometry analysis of bEND3 (mouse brain endothelial cell line) cells labeling CD31 with ab7388 at 10 µg/ml concentration. 106 cells were used per sample. No fixation or permeabilization needed. A R-PE labelled goat anti-rat IgG (H+L) was used as the secondary antibody.
Protocols
Datasheets and documents
References (82)
ab7388 has been referenced in 82 publications.
- Póvoa VCO et al. Wound healing action of nitric oxide-releasing self-expandable collagen sponge. J Tissue Eng Regen Med 14:807-818 (2020). PubMed: 32330363
- Tan T et al. Targeting peptide-decorated biomimetic lipoproteins improve deep penetration and cancer cells accessibility in solid tumor. Acta Pharm Sin B 10:529-545 (2020). PubMed: 32140397
- Sun L et al. Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair. Front Cell Dev Biol 8:263 (2020). PubMed: 32432109
- Liang X et al. Overexpression of ERBB4 rejuvenates aged mesenchymal stem cells and enhances angiogenesis via PI3K/AKT and MAPK/ERK pathways. FASEB J 33:4559-4570 (2019). PubMed: 30566395
- Gravina GL et al. The Small Molecule Ephrin Receptor Inhibitor, GLPG1790, Reduces Renewal Capabilities of Cancer Stem Cells, Showing Anti-Tumour Efficacy on Preclinical Glioblastoma Models. Cancers (Basel) 11:N/A (2019). PubMed: 30871240
