Recombinant

Recombinant Anti-CD31 antibody [MEC 7.46] (ab7388)

Reviews (8)Q&A (9)References (99)
Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (ab7388)
  • Immunocytochemistry - Anti-CD31 antibody [MEC 7.46] (ab7388)
  • Anti-CD31 antibody [MEC 7.46] (ab7388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [MEC 7.46] to CD31
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CD31 antibody [MEC 7.46]
    See all CD31 primary antibodies

  • Description

    Rat monoclonal [MEC 7.46] to CD31

  • Host species

    Rat

  • Specificity

    The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cell presents on all mouse blood vessels both in normal and inflamed or tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.

  • Tested applications

    Suitable for: Flow Cyt, ICCmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: mouse bEnd.3 cell line; Flow Cyt: mouse bEnd.3 cell line

  • General notes

    This product has switched from a hybridoma to recombinant production method on 08th March 2021.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/1000.
ICC
1/50.
Notes
Flow Cyt
1/1000.
ICC
1/50.
Application notes
Is unsuitable for WB.

Target

  • Function

    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

  • Tissue specificity

    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).

  • Sequence similarities

    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

  • Domain

    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.

  • Post-translational
    modifications

    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.

  • Cellular localization

    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt

  • Database links

    • Alternative names

      • Adhesion molecule antibody
      • CD31 antibody
      • CD31 antigen antibody
      • CD31 EndoCAM antibody
      • EndoCAM antibody
      • FLJ34100 antibody
      • FLJ58394 antibody
      • GPIIA antibody
      • GPIIA' antibody
      • PECA1 antibody
      • PECA1_HUMAN antibody
      • Pecam 1 antibody
      • PECAM 1 CD31 EndoCAM antibody
      • PECAM antibody
      • PECAM-1 antibody
      • Pecam1 antibody
      • Platelet and endothelial cell adhesion molecule 1 antibody
      • Platelet endothelial cell adhesion molecule antibody
      • Platelet/endothelial cell adhesion molecule 1 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (ab7388)
      Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (ab7388)

      Flow cytometric analysis of / fixed / permeabilized B16-F0 (Mouse melanoma epithelial cell-like, Left) / bEnd.3 (Mouse brain endothelioma, Right) cells labelling CD31 with ab7388 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control: B16-F0.Gated on viable cells.

    • Immunocytochemistry - Anti-CD31 antibody [MEC 7.46] (ab7388)
      Immunocytochemistry - Anti-CD31 antibody [MEC 7.46] (ab7388)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 cells labelling CD31 with ab7388 at 1/50 (19.32 µg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in bEnd.3 cell line. Negative control: B16-F0 (PMID: 8082649) ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Anti-CD31 antibody [MEC 7.46] (ab7388)
      Anti-CD31 antibody [MEC 7.46] (ab7388)

    References (99)

    Publishing research using ab7388? Please let us know so that we can cite the reference in this datasheet.

    ab7388 has been referenced in 99 publications.

    • Squecco R  et al. Bone Marrow-Mesenchymal Stromal Cell Secretome as Conditioned Medium Relieves Experimental Skeletal Muscle Damage Induced by Ex Vivo Eccentric Contraction. Int J Mol Sci 22:N/A (2021). PubMed: 33807453
    • Yokoe I  et al. Lipid bubbles combined with low-intensity ultrasound enhance the intratumoral accumulation and antitumor effect of pegylated liposomal doxorubicin in vivo. Drug Deliv 28:530-541 (2021). PubMed: 33685314
    • Zhu W  et al. Macrophage migration inhibitory factor facilitates the therapeutic efficacy of mesenchymal stem cells derived exosomes in acute myocardial infarction through upregulating miR-133a-3p. J Nanobiotechnology 19:61 (2021). PubMed: 33639970
    • Horiuchi K  et al. Pericyte-specific deletion of ninjurin-1 induces fragile vasa vasorum formation and enhances intimal hyperplasia of injured vasculature. Am J Physiol Heart Circ Physiol 320:H2438-H2447 (2021). PubMed: 33961504
    • Sommer K  et al. Local application reduces number of needed EPC for beneficial effects on wound healing compared to systemic treatment in mice. Eur J Trauma Emerg Surg N/A:N/A (2021). PubMed: 33813603
    View all Publications for this product

    Customer reviews and Q&As

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    1-10 of 17 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-CD31 antibody [MEC 7.46]

     Average
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (ear skin)
    Specification
    ear skin
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 28 2020

    Immunohistochemistry free floating abreview for Anti-CD31 antibody [MEC 7.46]

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue (Brain)
    Specification
    Brain
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 23 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-CD31 antibody [MEC 7.46]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (mouse tumor tissue)
    Permeabilization
    Yes - PBST(0.5% v/v triton X-100, sigma)
    Specification
    mouse tumor tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 21 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [MEC 7.46]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Adipose tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM citric acid, 55'C, 20min
    Permeabilization
    Yes - PBS/ 0.1% v/v Triton X-100 (Sigma)
    Specification
    Adipose tissue
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Bongju Park

    Verified customer

    Submitted Dec 01 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody [MEC 7.46]

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako target retrieval solution
    Permeabilization
    No
    Specification
    mouse skin
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 19 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-CD31 [MEC 7.46] antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E10.5 mouse embryos transverse section)
    Specification
    E10.5 mouse embryos transverse section
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - PBST (PBS / 0.5% v/v Triton X-100 (Sigma))
    Blocking step
    NOVOCASTRA protein block as blocking agent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 13 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-CD31 [MEC 7.46] antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (mouse skin)
    Specification
    mouse skin
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 09 2013

    Question

    Inquiry: Dear abcam-team I would like to draw your attention to one particular review submitted on 24 February 2011 in connection with the CD31-antibody ab7388. As the reviewer remained anonymous I put my question to you instead: The host of ab7388 is rat but the anonymous reviewer has used the DAKO Envision mouse polymer detection system and reported no staining. I have searched all DAKO Envision products and found that they react either with mouse and/ or with rabbit primary antibodies. This could explain those negative staining results. I am interested in purchasing ab7388 but am hesitating because of this review. Is there any chance that you could find out exactly what secondary antibody this anonymous reviewer has in fact used to detect ab7388 in his mouse tissue? I am looking forward to your reply. best regards

    Read More
    Answer

    I now have some further information to share in regards to the anti-CD31 antibody [MEC 7.46] (ab7388).

    I have been in contact with the reviewer and they have confirmed that they did indeed use an anti-mouse HRP DAKO kit to detect this rat antibody. This is likely to have yielded very poor results as the cross reactivity with rat is likely to have been poor and as thetissue being stained was from mouse the background was likely very high. Thank you for noticing this error and bringing it to our attention. I have now removed this review from the website to avoid further confusion.

    If I can help in any other way please do let me know. I wish you all the best with your research.

    Read More

    Question

    In the title of the attchament I said I used Cell cultures from bEnd3 cells. Yes, I though that the problem could be the secondary antibody but I bought it to abcam at the same time (the problem is the same) and to clarify this issue I need another primary of the same specie (rat) because I don't have it to test.   Thanks in advance,  

    Read More
    Answer

    Thank you for your response and for clarifying the cell types used in the experiments.    I understand now that bEnd3 cells (immortalized mouse brain endothelial cell line) were stained for CD31. The protocol looks absolutely fine and it you have an alternative antibody which worked fine with these cells. I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.   I could raise either a credit note for the cost of  one vial of ab7388 and ab7388 since you have alternative antibodies; or send you two replacement vials (one of each)  free of charge. Please could you let me know how you wish to proceed with your enquiry? I look forward to hearing from you soon.

    Read More

    Question

    I send in attach the protocol I used (that unswer to all questions of the inquiry). This protocol is optimized for a lot of antigens, namely CD31....   Fixative for 10 min at RT: PFA 4% Acetone PFA:Acetone (1:1) Wash 3x in PBS Permeabilize with Triton X-100 0,2%, 10 min, RT; Wash 3x in PBS Blockinh with BSA/PBS 1%, 40-60 min, RT; Wash in PBS Incubate 1st antibody, ON, 4ºC (rat CD31 abcam, ab7388) 1:50 1:100 1:500 1:1000 Wash in PBS Incubate with 2nd antibody in the dark, for 2h, RT (anti-rat abcam, ab6565) 1:1000 1:500 Wash in the dark Coverslip with Prolong Gold + DAPI. Fixative for 10 min at RT: PFA 4% Acetone PFA:Acetone (1:1) Wash 3x in PBS Permeabilize with Triton X-100 0,2%, 10 min, RT; Wash 3x in PBS Blockinh with BSA/PBS 1%, 40-60 min, RT; Wash in PBS Incubate 1st antibody, ON, 4ºC (rat CD31 abcam, ab7388) 1:50 1:100 1:500 1:1000 Wash in PBS Incubate with 2nd antibody in the dark, for 2h, RT (anti-rat abcam, ab6565) 1:1000 1:500 Wash in the dark Coverslip with Prolong Gold + DAPI.  

    Read More
    Answer

    Thank you for your response. Though you have kindly provided some details, it would be much appreciated if I could get some more information which would help me identify the source of the problem. 1)  Sample: Could you specify the samples used for the staining i.e. species, cells (cultured) or tissues? 2) Detection system: Have you used the secondary antibody successfully with another primary antibody? Do you think that the problem may come from the secondary antibody? Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

    Read More

    1-10 of 17 Abreviews or Q&A

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