Recombinant Anti-CD31 antibody [SP164] - C-terminal (ab227656)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP164] to CD31 - C-terminal
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD31 antibody [SP164] - C-terminal
See all CD31 primary antibodies -
Description
Rabbit monoclonal [SP164] to CD31 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (Intra): Jurkat cells. IHC-P: Human tonsil, cervix, placenta, lymph node, lung, skin, skin squamous cell carcinoma, lung squamous cell carcinoma, stomach adenocarcinoma, prostate adenocarcinoma and breast ductal carcinoma tissues.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP164 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/20 - 1/100.
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue |
Notes |
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Flow Cyt (Intra)
1/20 - 1/100. |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue |
Target
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Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
Tissue specificity
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
Sequence similarities
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
Post-translational
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
Cellular localization
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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Database links
- Entrez Gene: 5175 Human
- Entrez Gene: 18613 Mouse
- Omim: 173445 Human
- SwissProt: P16284 Human
- SwissProt: Q08481 Mouse
- Unigene: 376675 Human
- Unigene: 514412 Human
- Unigene: 343951 Mouse
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Alternative names
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
Images
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Flow cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) labeling CD31 with purified ab227656 at 1/20 dilution (5.6 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
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Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD31 with ab227656 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).
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Formalin-fixed, paraffin-embedded human cervix tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human skin tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for CD31 using ab227656 at 1/50 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227656 has not yet been referenced specifically in any publications.