Overview

  • Product name

    Anti-CD31 antibody [SP38]
    See all CD31 primary antibodies
  • Description

    Rabbit monoclonal [SP38] to CD31
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide within Mouse CD31 (C terminal). The exact sequence is proprietary.
    Database link: Q08481

  • Positive control

    • IHC-P: Human tonsil, endometrium, lung, lung adenocarcinoma, endometrial adenocarcinoma, liver, liver hepatocellular carcinoma, heart, colon, colon adenocarcinoma, cervix and esophagus tissue.

Applications

Our Abpromise guarantee covers the use of ab231436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.

Boil tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab231436 for 10 minutes at room temperature.

Target

  • Function

    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • Tissue specificity

    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • Sequence similarities

    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • Domain

    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • Post-translational
    modifications

    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • Cellular localization

    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human endometrium tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human heart tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma  tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervix tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human esophagus tissue stained for CD31 using ab231436 at 1/50 dilution in immunohistochemical analysis.

Protocols

References

This product has been referenced in:

  • Clement M  et al. Upholding the T cell immune-regulatory function of CD31 inhibits the formation of T/B immunological synapses in vitro and attenuates the development of experimental autoimmune arthritis in vivo. J Autoimmun 56:23-33 (2015). Read more (PubMed: 25277651) »
  • Cañadas I  et al. Targeting epithelial-to-mesenchymal transition with Met inhibitors reverts chemoresistance in small cell lung cancer. Clin Cancer Res 20:938-50 (2014). Read more (PubMed: 24284055) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Mouse Tissue (Cornea)
Specification
Cornea

Abcam user community

Verified customer

Submitted Aug 16 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris
Permeabilization
Yes - TBS-T
Specification
Mouse pancreas
Blocking step
1% BSA + 1% FBS in TBS-T as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 15 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Fibromatosis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris
Permeabilization
Yes - TBS-T
Specification
Fibromatosis
Blocking step
1% BSA + 1% FBS in TBS-T as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Mr. Héctor Franco

Verified customer

Submitted Feb 15 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris
Permeabilization
Yes - TBS-T
Specification
mouse pancreas
Blocking step
BSA / FBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 15 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human fibromatosis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9
Permeabilization
Yes - TBS-T
Specification
human fibromatosis
Blocking step
BSA / FBS as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 15 2019

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