Key features and details
- Mouse monoclonal [TLD-3A12] to CD31 (FITC)
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameAnti-CD31 antibody [TLD-3A12] (FITC)
See all CD31 primary antibodies
DescriptionMouse monoclonal [TLD-3A12] to CD31 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityAb33858 recognises CD31 expressed primarily on endothelial cells, platelets and leucocytes.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Rat
Does not react with: Human
Tissue, cells or virus corresponding to Rat CD31.
This clone (TLD 3A12) has been shown to partially block the proliferative response of antigen specific CD4+ T cells to antigen presenting cells and relevant antigen.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesAb33858 was purified from tissue culture supernatant.
Primary antibody notesThis clone (TLD 3A12) has been shown to partially block the proliferative response of antigen specific CD4+ T cells to antigen presenting cells and relevant antigen.
Our Abpromise guarantee covers the use of ab33858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells.
Use 10µl for labeling 106 cells in 100µl.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionInduces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Tissue specificityExpressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
Sequence similaritiesContains 6 Ig-like C2-type (immunoglobulin-like) domains.
DomainThe Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
Cellular localizationMembrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
- Information by UniProt
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
Figure A. RPE conjugated mouse anti rat TCRa/b and FITC conjugated mouse IgG1 isotype control. Figure B. RPE conjugated mouse anti rat TCRa/b and ab33858. All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Figure A. ab33858 and RPE conjugated mouse IgG1 isotype control. Figure B. ab33858 and RPE conjugated mouse anti rat TCRa/b. All experiments performed on red cell lysed rat blood gated on mononuclear cells.
ab33858 has been referenced in 16 publications.
- Shi L et al. Impaired function of tendon-derived stem cells in experimental diabetes mellitus rat tendons: implications for cellular mechanism of diabetic tendon disorder. Stem Cell Res Ther 10:27 (2019). PubMed: 30646947
- Lin Y et al. In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications. Int J Nanomedicine 14:5831-5848 (2019). PubMed: 31534327
- Zhu L et al. Spermine on Endothelial Extracellular Vesicles Mediates Smoking-Induced Pulmonary Hypertension Partially Through Calcium-Sensing Receptor. Arterioscler Thromb Vasc Biol 39:482-495 (2019). PubMed: 30626206
- Ai J et al. Generation of an anti-angiogenic endothelial progenitor cell line via endostatin gene transfer. Mol Med Rep 17:5814-5820 (2018). PubMed: 29484399
- Wang Y et al. A functional nanocarrier that copenetrates extracellular matrix and multiple layers of tumor cells for sequential and deep tumor autophagy inhibitor and chemotherapeutic delivery. Autophagy 13:359-370 (2017). PubMed: 27911136