Recombinant Anti-CD32 antibody [4F5] (ab235122)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD32 antibody [4F5]
See all CD32 primary antibodies -
Description
Mouse monoclonal [4F5] to CD32 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- FC: Raji cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4F5 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab235122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | 1/600. |
Target
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Function
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. -
Tissue specificity
Found on monocytes, neutrophils and eosinophil platelets. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2212 Human
- Entrez Gene: 2213 Human
- Entrez Gene: 9103 Human
- Omim: 146790 Human
- Omim: 604590 Human
- Omim: 612169 Human
- SwissProt: P12318 Human
- SwissProt: P31994 Human
see all -
Alternative names
- CD32 antibody
- CD32A antibody
- CD32B antibody
see all
Images
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Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cell line labeling CD32 with ab235122 at 1/600 dilution (red) compared with a Mouse IgG, monoclonal Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG ab150113 (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Datasheets and documents
References
ab235122 has not yet been referenced specifically in any publications.