Overview

  • Product name
    Anti-CD32 antibody [AT10] - BSA and Azide free
    See all CD32 primary antibodies
  • Description
    Mouse monoclonal [AT10] to CD32 - BSA and Azide free
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    K562 cell line

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab41899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use a concentration of 20 µg/ml. PubMed: 23454746
IP Use a concentration of 20 µg/ml.

Target

  • Function
    Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
  • Tissue specificity
    Found on monocytes, neutrophils and eosinophil platelets.
  • Sequence similarities
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD32 antibody
    • CD32A antibody
    • CD32B antibody
    • CD32C antibody
    • CDw32 antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
    • Fc fragment of IgG low affinity IIc receptor antibody
    • Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
    • Fc gamma receptor IIB antibody
    • Fc gamma receptor IIC antibody
    • Fc gamma RII a antibody
    • Fc gamma RII b antibody
    • Fc gamma RII c antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIIc antibody
    • Fc receptor antibody
    • Fc receptor, IgG, low affinity IIb antibody
    • Fc-gamma RII-a antibody
    • Fc-gamma RII-b antibody
    • Fc-gamma RII-c antibody
    • Fc-gamma-RIIa antibody
    • FC-gamma-RIIB antibody
    • Fc-GAMMA-RIIC antibody
    • Fc[g]RII antibody
    • FCG2 antibody
    • FCG2A_HUMAN antibody
    • FcGR antibody
    • FCGR2 antibody
    • FCGR2A antibody
    • FCGR2A1 antibody
    • FCGR2B antibody
    • FCGR2C antibody
    • Fcgr3 antibody
    • Fcgr3a antibody
    • FcgRII antibody
    • Fcr-2 antibody
    • Fcr-3 antibody
    • FcRII a antibody
    • FCRII antibody
    • FcRII b antibody
    • FcRII c antibody
    • FcRII-a antibody
    • FcRII-b antibody
    • FcRIIC antibody
    • IGFR2 antibody
    • IgG Fc receptor II a antibody
    • IgG Fc receptor II b antibody
    • IgG Fc receptor II beta antibody
    • IgG Fc receptor II c antibody
    • IgG Fc receptor II-a antibody
    • IgG Fc receptor II-b antibody
    • IgG Fc receptor II-c antibody
    • Immunoglobulin G Fc receptor II antibody
    • Immunoglobulin G Fc receptor II c antibody
    • Low affinity immunoglobulin gamma Fc region receptor II antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-c antibody
    • Ly-17 antibody
    • Ly-m20 antibody
    • Lym 1 antibody
    • LyM-1 antibody
    • Lymphocyte antigen 17 antibody
    see all

Images

  • Overlay histogram showing K562 cells stained with ab41899 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41899, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed K562 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:
  • Karagiannis P  et al. IgG4 subclass antibodies impair antitumor immunity in melanoma. J Clin Invest 123:1457-74 (2013). IHC-Fr ; Human . Read more (PubMed: 23454746) »
  • Municio C  et al. The response of human macrophages to ß-glucans depends on the inflammatory milieu. PLoS One 8:e62016 (2013). IP ; Human . Read more (PubMed: 23637950) »
See all 11 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

The IP protcol could be found at the following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11385

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you very much for your interest in ab41899 Anti-CD32 antibody [AT10] - Azide free.

According to Bunk S et al., this antibody completly blocksFcγRII (CD32) in human PBMCs.Please see the attachment.

To our knowledge, ab41899 has not been tested in ELISA. Therefore, I can offer a discount off a future purchase if you buy ab41899 now, test it in ELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab41899 in ELISA. I will then send a discount code. This code must be issued before purchasing ab41899 so please wait for my reply before ordering.

2. Purchase ab41899 either by phone, fax, or online (www.abcam.com).

3. Test it in ELISA.


4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab41899 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you very much for your interest in our products. ab89191 (Anti-CD32 antibody [MM0270- 3L1]) from our catalogue was raised using CD32b extra cellular domain and reacts with both CD32a and CD32b. Unfortunately, it was not specifically tested if it reacts with CD32c. But since the CD32c and CD32b extra cellular domains are identical, we assume that it should also recognize CD32c. This antibody is tested and guaranteed to work in WB with human samples by our Abpromise guarantee. Please follow this link to review the datasheet of ab89191: Click here (or use the following: https://www.abcam.com/index.html?datasheet=89191). I am also happy to confirm that ab41899 (Anti-CD32 antibody [AT10]) binds to the extracellular region of CD32 and reacts with all isoforms of CD32, as described in Sandilands, G.P. et al. (2007) Immunol. 91: 204-211. Furthermore, clone AT10 has been shown to block CD32 and displace any antigen (IgG) that is bound to the receptor. This antibody is not tested and guaranteed for WB. If you would like to test this antibody in WB you might be eligible for our Abreview testing discount program. I hope this information was helpful. Please do not hesitate to contact me again with any further questions or if you would like to test ab41899 in WB.

Read More

Answer

Thank you for your enquiry. I can confirm the following details which I have obtained from the laboratories: ab41899 CD32 antibody: Unfortunately, we do not have a specific protocol for the use of this product in blocking experiments, this application is supported based upon the literature. There are a number of articles which may be helpful to you, I have listed some examples below: Larsson M, Berge J, Johansson AG, Forsum U. Human dendritic cells handling of binding, uptake and degradation of free and IgG-immune complexed dinitrophenylated human serum albumin in vitro. Immunology. 1997 Jan;90(1):138-46. PMID: 9038724 Bredius RG, Fijen CA, De Haas M, Kuijper EJ, Weening RS, Van de Winkel JG, Out TA. Role of neutrophil Fc gamma RIIa (CD32) and Fc gamma RIIIb (CD16) polymorphic forms in phagocytosis of human IgG1- and IgG3-opsonized bacteria and erythrocytes. Immunology. 1994 Dec;83(4):624-30. PMID: 7875742 Pawar P, Shin PK, Mousa SA, Ross JM, Konstantopoulos K. Fluid shear regulates the kinetics and receptor specificity of Staphylococcus aureus binding to activated platelets. J Immunol. 2004 Jul 15;173(2):1258-65. PMID: 15240718 ab46679 CD16 antibody This antibody can be used for blocking IgG binding to FcγRIII. Further details can be found in the following references: HLDA IV - PART 4: NK13.3 section - NK cell structural studies: Localization of the epitopes of hu CD16 MAbs on the molecular model of CD16, Tamm, A., et al., p. 324-326. Tamm A, Schmidt RE.: The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 1996 Aug 15;157(4):1576-81. PMID: 8759741 I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again.

Read More

Question
Answer

As far as I am aware, there has not been a study formally identifying the minimal epitoperecognised by this antibody. However, there is some information on the epitope recognised in the original paper describing the antibody: Greenman J, Tutt AL, George AJ, Pulford KA, Stevenson GT, Glennie MJ. Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors. Mol Immunol. 1991 Nov;28(11):1243-54. Metes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA. Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood. 1998 Apr 1;91(7):2369-80. I hope that is of some help to you,

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