Product nameAnti-CD32 antibody [EP6243]
See all CD32 primary antibodies
DescriptionRabbit monoclonal [EP6243] to CD32
SpecificityBased on the immunogen sequence, this antibody may show cross reactivity with CD32 A, B and C.
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human CD32 aa 1-100 (extracellular). The exact sequence is proprietary.
- Daudi, U937, Raji, and Human pancreas lysates; Human spleen tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.40 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 37-43 kDa (predicted molecular weight: 34 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBinds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
Tissue specificityFound on monocytes, neutrophils and eosinophil platelets.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
- CD32 antibody
- CD32A antibody
- CD32B antibody
Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD32 with ab131051 antibody at 1/100 dilution.
All lanes : Anti-CD32 antibody [EP6243] (ab131051) at 1/10000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Human pancreas lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Actual band size : 37-43 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131051 has not yet been referenced specifically in any publications.