Overview

  • Product name

    Anti-CD32 antibody [EPR6657(2)]
    See all CD32 primary antibodies
  • Description

    Rabbit monoclonal [EPR6657(2)] to CD32
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD32. The exact sequence is proprietary.
    (Peptide available as ab211774)

  • Positive control

    • WB: K562, U937, Daudi, human placenta and human platelet lysates. IHC-P: Human stomach and tonsil tissues.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37-42 kDa (predicted molecular weight: 35 kDa).Can be blocked with CD32 peptide (ab211774).
IHC-P 1/1000.

See IHC antigen retrieval protocols.

For unpurified format use at 1/250 - 1/500 dilution.

Target

  • Function

    Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
  • Tissue specificity

    Found on monocytes, neutrophils and eosinophil platelets.
  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD32 antibody
    • CD32A antibody
    • CD32B antibody
    • CD32C antibody
    • CDw32 antibody
    • Fc fragment of IgG low affinity IIa receptor antibody
    • Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
    • Fc fragment of IgG low affinity IIb receptor antibody
    • Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
    • Fc fragment of IgG low affinity IIc receptor antibody
    • Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
    • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
    • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
    • Fc gamma receptor IIB antibody
    • Fc gamma receptor IIC antibody
    • Fc gamma RII a antibody
    • Fc gamma RII b antibody
    • Fc gamma RII c antibody
    • Fc gamma RIIb antibody
    • Fc gamma RIIc antibody
    • Fc receptor antibody
    • Fc receptor, IgG, low affinity IIb antibody
    • Fc-gamma RII-a antibody
    • Fc-gamma RII-b antibody
    • Fc-gamma RII-c antibody
    • Fc-gamma-RIIa antibody
    • FC-gamma-RIIB antibody
    • Fc-GAMMA-RIIC antibody
    • Fc[g]RII antibody
    • FCG2 antibody
    • FCG2A_HUMAN antibody
    • FcGR antibody
    • FCGR2 antibody
    • FCGR2A antibody
    • FCGR2A1 antibody
    • FCGR2B antibody
    • FCGR2C antibody
    • Fcgr3 antibody
    • Fcgr3a antibody
    • FcgRII antibody
    • Fcr-2 antibody
    • Fcr-3 antibody
    • FcRII a antibody
    • FCRII antibody
    • FcRII b antibody
    • FcRII c antibody
    • FcRII-a antibody
    • FcRII-b antibody
    • FcRIIC antibody
    • IGFR2 antibody
    • IgG Fc receptor II a antibody
    • IgG Fc receptor II b antibody
    • IgG Fc receptor II beta antibody
    • IgG Fc receptor II c antibody
    • IgG Fc receptor II-a antibody
    • IgG Fc receptor II-b antibody
    • IgG Fc receptor II-c antibody
    • Immunoglobulin G Fc receptor II antibody
    • Immunoglobulin G Fc receptor II c antibody
    • Low affinity immunoglobulin gamma Fc region receptor II antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
    • Low affinity immunoglobulin gamma Fc region receptor II-c antibody
    • Ly-17 antibody
    • Ly-m20 antibody
    • Lym 1 antibody
    • LyM-1 antibody
    • Lymphocyte antigen 17 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD32 with purified ab155972 at 1/1000 dilution (0.98 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Anti-CD32 antibody [EPR6657(2)] (ab155972) at 1/10000 dilution (Purified) + U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa
    Observed band size: 37-42 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-CD32 antibody [EPR6657(2)] (ab155972) at 1/1000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : Daudi cell lysate
    Lane 4 : Human placenta lysate
    Lane 5 : Human platelet lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 35 kDa
    Observed band size: 37-42 kDa why is the actual band size different from the predicted?

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling CD32 with ab155972 (unpurified) at 1/250 dilution

  • ab155972 (unpurified) showing -ve staining in Human skeletal muscle.

  • ab155972 (unpurified) showing +ve staining in Human placenta.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD32 with ab155972 (unpurified) at 1/250 dilution

References

This product has been referenced in:

  • Cao J  et al. Versatile and on-demand biologics co-production in yeast. Nat Commun 9:77 (2018). WB . Read more (PubMed: 29311542) »
  • Sips M  et al. Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies. Mucosal Immunol 9:1584-1595 (2016). IHC ; Human . Read more (PubMed: 26883728) »
See all 2 Publications for this product

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