Recombinant Anti-CD32A + CD32C antibody [EPR6658] (ab125013)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6658] to CD32A + CD32C
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD32A + CD32C antibody [EPR6658] -
Description
Rabbit monoclonal [EPR6658] to CD32A + CD32C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CD32A aa 250-350. The exact sequence is proprietary.
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Positive control
- K562 and Human platelet lysates; Human lung and spleen tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.90 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6658 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 37-43 kDa (predicted molecular weight: 35 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 37-43 kDa (predicted molecular weight: 35 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Cellular localization
CD32A: Cell membrane. CD32C: Cytoplasm and Cell membrane. -
Database links
- Entrez Gene: 2212 Human
- Entrez Gene: 9103 Human
- Omim: 146790 Human
- SwissProt: P12318 Human
- SwissProt: P31995 Human
- Unigene: 352642 Human
- Unigene: 654395 Human
Images
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ab125013 at 1/250 staining CD32A + CD32C in paraffin-embedded Human lung tissue by immunohistochemistry. Heat mediated antigen retrieval was perrformed before commencing with IHC staining protocol.
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All lanes : Anti-CD32A + CD32C antibody [EPR6658] (ab125013) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : Human platelet lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 37-43 kDa why is the actual band size different from the predicted? -
ab125013 at 1/250 staining CD32A + CD32C in paraffin-embedded Human spleen tissue by immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab125013 has been referenced in 2 publications.
- McGuinness D et al. A molecular signature for delayed graft function. Aging Cell 17:e12825 (2018). PubMed: 30094915
- Murinello S et al. Fc? receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration. Invest Ophthalmol Vis Sci 55:247-58 (2014). IHC-P ; Human . PubMed: 24334446