Overview

  • Product name

    Anti-CD32 (phospho Y292) antibody [EP926Y]
    See all CD32 primary antibodies
  • Description

    Rabbit monoclonal [EP926Y] to CD32 (phospho Y292)
  • Host species

    Rabbit
  • Specificity

    Detects CD32b only when phosphorylated on Tyrosine 292.
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding tyrosine 292 of Human CD32

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab68423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa).
IP 1/70.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
    • Tissue specificity

      Found on monocytes, neutrophils and eosinophil platelets.
    • Sequence similarities

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD32 antibody
      • CD32A antibody
      • CD32B antibody
      • CD32C antibody
      • CDw32 antibody
      • Fc fragment of IgG low affinity IIa receptor antibody
      • Fc fragment of IgG low affinity IIa receptor for (CD32) antibody
      • Fc fragment of IgG low affinity IIb receptor antibody
      • Fc fragment of IgG low affinity IIb receptor for (CD32) antibody
      • Fc fragment of IgG low affinity IIc receptor antibody
      • Fc fragment of IgG low affinity IIc receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity II, receptor for (CD32) antibody
      • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
      • Fc fragment of IgG, low affinity IIb, receptor (CD32) antibody
      • Fc gamma receptor IIB antibody
      • Fc gamma receptor IIC antibody
      • Fc gamma RII a antibody
      • Fc gamma RII b antibody
      • Fc gamma RII c antibody
      • Fc gamma RIIb antibody
      • Fc gamma RIIc antibody
      • Fc receptor antibody
      • Fc receptor, IgG, low affinity IIb antibody
      • Fc-gamma RII-a antibody
      • Fc-gamma RII-b antibody
      • Fc-gamma RII-c antibody
      • Fc-gamma-RIIa antibody
      • FC-gamma-RIIB antibody
      • Fc-GAMMA-RIIC antibody
      • Fc[g]RII antibody
      • FCG2 antibody
      • FCG2A_HUMAN antibody
      • FcGR antibody
      • FCGR2 antibody
      • FCGR2A antibody
      • FCGR2A1 antibody
      • FCGR2B antibody
      • FCGR2C antibody
      • Fcgr3 antibody
      • Fcgr3a antibody
      • FcgRII antibody
      • Fcr-2 antibody
      • Fcr-3 antibody
      • FcRII a antibody
      • FCRII antibody
      • FcRII b antibody
      • FcRII c antibody
      • FcRII-a antibody
      • FcRII-b antibody
      • FcRIIC antibody
      • IGFR2 antibody
      • IgG Fc receptor II a antibody
      • IgG Fc receptor II b antibody
      • IgG Fc receptor II beta antibody
      • IgG Fc receptor II c antibody
      • IgG Fc receptor II-a antibody
      • IgG Fc receptor II-b antibody
      • IgG Fc receptor II-c antibody
      • Immunoglobulin G Fc receptor II antibody
      • Immunoglobulin G Fc receptor II c antibody
      • Low affinity immunoglobulin gamma Fc region receptor II antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-b antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-c antibody
      • Ly-17 antibody
      • Ly-m20 antibody
      • Lym 1 antibody
      • LyM-1 antibody
      • Lymphocyte antigen 17 antibody
      see all

    Images

    • All lanes : Anti-CD32 (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution

      Lane 1 : Untreated Daudi cell lysate
      Lane 2 : Pervanadate-treated Daudi cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

      Predicted band size: 40,44 kDa

    References

    This product has been referenced in:

    • Rieth N  et al. Characterization of SM201, an anti-hFc?RIIB antibody not interfering with ligand binding that mediates immune complex dependent inhibition of B cells. Immunol Lett 160:145-50 (2014). Read more (PubMed: 24472604) »
    • Sundgren NC  et al. Coupling of fc? receptor I to fc? receptor IIb by SRC kinase mediates C-reactive protein impairment of endothelial function. Circ Res 109:1132-40 (2011). Read more (PubMed: 21940940) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Question
    Answer

    Vielen Dank für Ihren Anruf.

    Ich kann Ihnen nun bestätigen, dass beide Antikörper spezifisch die IIb Isoform (und alle Unterformen von dieser Isoform: IIb1, IIb2, IIb3) binden.

    Beide Immunogen sind Peptide die die folgende Sequenz einschließt: TITYpSLL.

    Die Antikörper wurden nicht experimentell mit Isoform IIa und IIc getestet. Aber diese beiden Isoformen enthalten die Immunogensequenz TITYpSLL nicht.

    Ich hoffe, diese Information ist hilfreich und wünsche Ihnen viel Erfolg mit Ihrer Forschung.

    Read More

    Question
    Answer

    ab68423 detects CD32b and is not expected to cross-react with CD32a. However, this has not been specifically tested in the laboratory as far as I am aware.

    Read More

    Answer

    Thank you very much for contacting us with your question.

    I apologize for the confusing discrepancy on the Abcam and Epitomics datasheets. I've gotten in touch with the Epitomics lab, and they did test the antibody once on mouse samples (brain, heart, kidney and spleen) at a 1:100,000 primary dilution but did not observe a signal. They still recommend the product for mouse reactivity based on the fact that the immunogen sequence has 100% homology with the mouse protein.

    Though Epitomics is now an Abcam company, we are still working to merge all of our policies. Abcam only lists a species as reactive if it has actually been tested and shown positive results. If the results were negative then we say the antibody does not react with that species.

    Due to the above information, I hesitate to recommend this antibody for use with mouse samples. There may be tweaks to the protocol that would produce a signal with mouse lysates (such as increasing the concentration, 1:100,000 is quite high) but we can not guarantee this antibody to work with mouse.

    I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help. Have a nice day.

    Read More

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