Product nameAnti-CD32 (phospho Y292) antibody [EP926Y]
See all CD32 primary antibodies
DescriptionRabbit monoclonal [EP926Y] to CD32 (phospho Y292)
SpecificityDetects CD32b only when phosphorylated on Tyrosine 292.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic phosphopeptide corresponding to residues surrounding tyrosine 292 of Human CD32
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab68423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/100000. Detects a band of approximately 40,44 kDa (predicted molecular weight: 40,44 kDa).|
FunctionBinds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.
Tissue specificityFound on monocytes, neutrophils and eosinophil platelets.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
- CD32 antibody
- CD32A antibody
- CD32B antibody
All lanes : Anti-CD32 (phospho Y292) antibody [EP926Y] (ab68423) at 1/100000 dilution
Lane 1 : Untreated Daudi cell lysate
Lane 2 : Pervanadate-treated Daudi cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution
Predicted band size: 40,44 kDa
This product has been referenced in:
- Rieth N et al. Characterization of SM201, an anti-hFc?RIIB antibody not interfering with ligand binding that mediates immune complex dependent inhibition of B cells. Immunol Lett 160:145-50 (2014). Read more (PubMed: 24472604) »
- Sundgren NC et al. Coupling of fc? receptor I to fc? receptor IIb by SRC kinase mediates C-reactive protein impairment of endothelial function. Circ Res 109:1132-40 (2011). Read more (PubMed: 21940940) »