Product nameAnti-CD32A + CD32C antibody [EPR6658]
DescriptionRabbit monoclonal [EPR6658] to CD32A + CD32C
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human CD32A aa 250-350. The exact sequence is proprietary.
- K562 and Human platelet lysates; Human lung and spleen tissues.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 8.90 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab125013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 37-43 kDa (predicted molecular weight: 35 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Cellular localizationCD32A: Cell membrane. CD32C: Cytoplasm and Cell membrane.
ab125013 at 1/250 staining CD32A + CD32C in paraffin-embedded Human lung tissue by immunohistochemistry. Heat mediated antigen retrieval was perrformed before commencing with IHC staining protocol.
All lanes : Anti-CD32A + CD32C antibody [EPR6658] (ab125013) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : Human platelet lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 37-43 kDa why is the actual band size different from the predicted?
ab125013 at 1/250 staining CD32A + CD32C in paraffin-embedded Human spleen tissue by immunohistochemistry. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- McGuinness D et al. A molecular signature for delayed graft function. Aging Cell 17:e12825 (2018). Read more (PubMed: 30094915) »
- Murinello S et al. Fc? receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration. Invest Ophthalmol Vis Sci 55:247-58 (2014). IHC-P ; Human . Read more (PubMed: 24334446) »