Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [4F5] to CD32A
- Suitable for: Flow Cyt
- Reacts with: Human
Product nameAnti-CD32A antibody [4F5]
See all CD32A primary antibodies
DescriptionMouse monoclonal [4F5] to CD32A
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB
Species reactivityReacts with: Human
- Flow Cyt: Raji cells.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab235122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1.035 µg/ml.|
FunctionBinds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Tissue specificityFound on monocytes, neutrophils and eosinophil platelets.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
modificationsPhosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
Cellular localizationCell membrane.
- Information by UniProt
- CD32 antibody
- CD32 antigen antibody
- CDw32 antibody
Flow cytometric analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cell line labeling CD32A with ab235122 at 1.035µg/ml (red) compared with a Mouse IgG, monoclonal Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG ab150113 (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235122 has not yet been referenced specifically in any publications.