Key features and details
- Rabbit polyclonal to CD32A
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CD32A antibody
See all CD32A primary antibodies
DescriptionRabbit polyclonal to CD32A
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human CD32A aa 241-317.
Database link: P12318
- WB: Mouse spleen tissue lysate. IHC-P: Human gastric cancer and kidney tissues. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab223039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 35 kDa.|
FunctionBinds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Tissue specificityFound on monocytes, neutrophils and eosinophil platelets.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
modificationsPhosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
Cellular localizationCell membrane.
- Information by UniProt
- CD32 antibody
- CD32 antigen antibody
- CDw32 antibody
Paraffin-embedded human gastric cancer tissue stained for CD32A using ab223039 at 1/100 dilution in immunohistochemical analysis.
Anti-CD32A antibody (ab223039) at 1/1000 dilution + Mouse spleen tissue lysate
Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Paraffin-embedded human kidney tissue stained for CD32A using ab223039 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD34A (green) using ab223039 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
ab223039 has not yet been referenced specifically in any publications.