Anti-CD32A antibody [OTI9C6] (ab124408)
Key features and details
- Mouse monoclonal [OTI9C6] to CD32A
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD32A antibody [OTI9C6]
See all CD32A primary antibodies -
Description
Mouse monoclonal [OTI9C6] to CD32A -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CD32A.
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Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY CD32; Human Breast, Colon, Kidney, Lung, Ovary, Ovary adenocarcinoma, Pancreas, Thyroid, Thyroid carcinoma, Endometrium and Prostate tissues; COS7 cells transiently transfected with pCMV6-ENTRY CD32;
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General notes
Dilute in PBS (pH7.3) before use. Avoid repeated freeze-thaws.
The clone number has been updated from 9C6 to OTI9C6, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI9C6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/2000. Predicted molecular weight: 35 kDa. | |
IHC-P | 1/50. | |
ICC/IF | 1/100. | |
IP | Use a concentration of 4 µg/ml. |
Target
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Function
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. -
Tissue specificity
Found on monocytes, neutrophils and eosinophil platelets. -
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2212 Human
- Omim: 146790 Human
- SwissProt: P12318 Human
- Unigene: 352642 Human
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Alternative names
- CD32 antibody
- CD32 antigen antibody
- CDw32 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human breast tissue by Immunohistochemistry.
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All lanes : Anti-CD32A antibody [OTI9C6] (ab124408) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD32
Lysates/proteins at 5 µg per lane.
Predicted band size: 35 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205503) -
Immunoprecipitation of CD32A. Lane 1: Negative control, no IgG. Lane2: 2µg of ab124408 and 20µl (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed with lysate and incubated overnight. For each experiment, 500µl of DDK tagged CD32 overexpression lysates (at 1:5 dilution with HEK293T lysate) was used. Predicted band size : 35 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human colon tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human kidney tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human lung tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human prostate tissue by Immunohistochemistry.
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ab124408, at 1/100 dilution, staining CD32A in COS7 cells transiently transfected with pCMV6-ENTRY CD32 by Immunofluorescence.
Protocols
References (0)
ab124408 has not yet been referenced specifically in any publications.