Anti-CD32A antibody [OTI9C6] (ab124408)

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Overview

  • Product name

    Anti-CD32A antibody [OTI9C6]
    See all CD32A primary antibodies

  • Description

    Mouse monoclonal [OTI9C6] to CD32A

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human CD32A.

  • Positive control

    • HEK293T cell lysate transfected with pCMV6-ENTRY CD32; Human Breast, Colon, Kidney, Lung, Ovary, Ovary adenocarcinoma, Pancreas, Thyroid, Thyroid carcinoma, Endometrium and Prostate tissues; COS7 cells transiently transfected with pCMV6-ENTRY CD32;

  • General notes

    Dilute in PBS (pH7.3) before use. Avoid repeated freeze-thaws.

    The clone number has been updated from 9C6 to OTI9C6, both clone numbers name the same antibody clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab124408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 35 kDa.
IHC-P 1/50.
ICC/IF 1/100.
IP Use a concentration of 4 µg/ml.

Target

  • Function

    Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.

  • Tissue specificity

    Found on monocytes, neutrophils and eosinophil platelets.

  • Sequence similarities

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • Post-translational
    modifications

    Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD32 antibody
      • CD32 antigen antibody
      • CDw32 antibody
      • Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
      • Fc fragment of IgG, low affinity IIa, receptor for (CD32) antibody
      • Fc gamma RII a antibody
      • Fc gamma RIIa antibody
      • Fc-gamma RII-a antibody
      • Fc-gamma-RIIa antibody
      • FCG2 antibody
      • FCG2A_HUMAN antibody
      • FcGR antibody
      • FCGR2 antibody
      • FCGR2A antibody
      • FCGR2A1 antibody
      • FcRII a antibody
      • FcRII-a antibody
      • IGFR2 antibody
      • IgG Fc receptor II a antibody
      • IgG Fc receptor II-a antibody
      • Immunoglobulin G Fc receptor II antibody
      • low affinity immunoglobulin gamma Fc region receptor II a antibody
      • Low affinity immunoglobulin gamma Fc region receptor II-a antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human breast tissue by Immunohistochemistry.

    • Western blot - Anti-CD32A antibody [OTI9C6] (ab124408)
      Western blot - Anti-CD32A antibody [OTI9C6] (ab124408)
      All lanes : Anti-CD32A antibody [OTI9C6] (ab124408) at 1/2000 dilution

      Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
      Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD32

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 35 kDa



      HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205503)
    • Immunoprecipitation - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunoprecipitation - Anti-CD32A antibody [OTI9C6] (ab124408)

      Immunoprecipitation of CD32A. Lane 1: Negative control, no IgG. Lane2: 2µg of ab124408 and 20µl (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed with lysate and incubated overnight. For each experiment, 500µl of DDK tagged CD32 overexpression lysates (at 1:5 dilution with HEK293T lysate) was used. Predicted band size : 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human colon tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human lung tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human pancreas tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human endometrium tissue by Immunohistochemistry.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human prostate tissue by Immunohistochemistry.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD32A antibody [OTI9C6] (ab124408)
      Immunocytochemistry/ Immunofluorescence - Anti-CD32A antibody [OTI9C6] (ab124408)

      ab124408, at 1/100 dilution, staining CD32A in COS7 cells transiently transfected with pCMV6-ENTRY CD32 by Immunofluorescence.

    References

    ab124408 has not yet been referenced specifically in any publications.

    Publishing research using ab124408? Please let us know so that we can cite the reference in this datasheet.

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