Anti-CD32A antibody [OTI9C6] (ab124408)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

Key features and details

Mouse monoclonal [OTI9C6] to CD32A
Suitable for: WB, IHC-P, ICC/IF, IP
Reacts with: Human
Isotype: IgG1

Product name

Anti-CD32A antibody [OTI9C6]
See all CD32A primary antibodies
Description

Mouse monoclonal [OTI9C6] to CD32A
Host species

Mouse
Tested applications

Suitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant full length protein corresponding to Human CD32A.
Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY CD32; Human Breast, Colon, Kidney, Lung, Ovary, Ovary adenocarcinoma, Pancreas, Thyroid, Thyroid carcinoma, Endometrium and Prostate tissues; COS7 cells transiently transfected with pCMV6-ENTRY CD32;
General notes

Dilute in PBS (pH7.3) before use. Avoid repeated freeze-thaws.

The clone number has been updated from 9C6 to OTI9C6, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from TCS
Clonality

Monoclonal
Clone number

OTI9C6
Isotype

IgG1
Research areas

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Recombinant Protein
- Recombinant human CD32A protein (ab207140)

Our Abpromise guarantee covers the use of ab124408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/2000. Predicted molecular weight: 35 kDa.
IHC-P		1/50.
ICC/IF		1/100.
IP		Use a concentration of 4 µg/ml.

Function

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Tissue specificity

Found on monocytes, neutrophils and eosinophil platelets.
Sequence similarities

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Post-translational
modifications

Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P12318 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2212 Human
- Omim: 146790 Human
- SwissProt: P12318 Human
- Unigene: 352642 Human
Alternative names
- CD32 antibody
- CD32 antigen antibody
- CDw32 antibody
- Fc fragment of IgG, low affinity IIa, receptor (CD32) antibody
- Fc fragment of IgG, low affinity IIa, receptor for (CD32) antibody
- Fc gamma RII a antibody
- Fc gamma RIIa antibody
- Fc-gamma RII-a antibody
- Fc-gamma-RIIa antibody
- FCG2 antibody
- FCG2A_HUMAN antibody
- FcGR antibody
- FCGR2 antibody
- FCGR2A antibody
- FCGR2A1 antibody
- FcRII a antibody
- FcRII-a antibody
- IGFR2 antibody
- IgG Fc receptor II a antibody
- IgG Fc receptor II-a antibody
- Immunoglobulin G Fc receptor II antibody
- low affinity immunoglobulin gamma Fc region receptor II a antibody
- Low affinity immunoglobulin gamma Fc region receptor II-a antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human breast tissue by Immunohistochemistry.
Western blot - Anti-CD32A antibody [OTI9C6] (ab124408)

All lanes : Anti-CD32A antibody [OTI9C6] (ab124408) at 1/2000 dilution

Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD32

Lysates/proteins at 5 µg per lane.

Predicted band size: 35 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205503)
Immunoprecipitation - Anti-CD32A antibody [OTI9C6] (ab124408)

Immunoprecipitation of CD32A. Lane 1: Negative control, no IgG. Lane2: 2µg of ab124408 and 20µl (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed with lysate and incubated overnight. For each experiment, 500µl of DDK tagged CD32 overexpression lysates (at 1:5 dilution with HEK293T lysate) was used. Predicted band size : 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human colon tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human lung tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/50 dilution, staining CD32A in paraffin-embedded Human prostate tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-CD32A antibody [OTI9C6] (ab124408)

ab124408, at 1/100 dilution, staining CD32A in COS7 cells transiently transfected with pCMV6-ENTRY CD32 by Immunofluorescence.

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Publishing research using ab124408? Please let us know so that we can cite the reference in this datasheet.

ab124408 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Anti-CD32A antibody [OTI9C6] (ab124408)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

Target

Function

Tissue specificity

Sequence similarities

Post-translational
modifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (0)

Customer reviews and Q&As

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References (0)

Customer reviews and Q&As

Post-translational
modifications