Product nameAnti-CD32B + CD32A + CD32C antibody [EP6243]
DescriptionRabbit monoclonal [EP6243] to CD32B + CD32A + CD32C
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human CD32A aa 1-100 (extracellular). The exact sequence is proprietary.
- Daudi, U937, Raji, and Human pancreas lysates; Human spleen tissue. ICC/IF: Daudi cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.40 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab131051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 37-43 kDa (predicted molecular weight: 34 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Cellular localizationCD32B: Cell membrane. CD32A: Cell membrane. CD32C: Cytoplasm and Cell membrane.
Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD32B + CD32A + CD32C with ab131051 antibody at 1/100 dilution. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence analysis of Daudi (human Burkitt's lymphoma lymphoblast) cells labeling CD32B + CD32A + CD32C with purified ab131051 at 1/500 dilution (4.6 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.
All lanes : Anti-CD32B + CD32A + CD32C antibody [EP6243] (ab131051) at 1/10000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Human pancreas lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Actual band size : 37-43 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131051 has not yet been referenced specifically in any publications.